IF (Immunofluorescence)
(Confocal immunofluorescence analysis of U2OS cells using TOMM40 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.)
Rabbit TOMM40 Polyclonal Antibody | anti-TOMM40 antibody
TOMM40 Polyclonal Antibody
Gene Names
TOMM40; TOM40; PEREC1; C19orf1; PER-EC1; D19S1177E
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Affinity Purification
Synonyms
TOMM40, Antibody; TOMM40 Polyclonal Antibody; tomm40: TOM40; PEREC1; C19orf1; PER-EC1; D19S1177E; anti-TOMM40 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Sequence Length
361
Applicable Applications for anti-TOMM40 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF/ICC: 1:50 - 1:200
IHC: 1:50 - 1:200
IF/ICC: 1:50 - 1:200
Cross-Reactivity
Human, Mouse, Rat
Conjugation
Unconjugated
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human TOMM40 (NP_001122389.1).
Preparation and Storage
Store at -20°C. Avoid freeze / thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescence analysis of U2OS cells using TOMM40 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Confocal immunofluorescence analysis of U2OS cells using TOMM40 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using TOMM40 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using TOMM40 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
Related Product Information for anti-TOMM40 antibody
The protein encoded by this gene is localized in the outer membrane of the mitochondria. It is the channel-forming subunit of the translocase of the mitochondrial outer membrane (TOM) complex that is essential for import of protein precursors into mitochondria. Alternatively spliced transcript variants have been found for this gene.
Product Categories/Family for anti-TOMM40 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Observed MW: 38kDa
Calculated MW: 38kDa
Calculated MW: 38kDa
NCBI Official Full Name
Mitochondrial import receptor subunit TOM40 homolog
NCBI Official Synonym Full Names
translocase of outer mitochondrial membrane 40 homolog (yeast)
NCBI Official Symbol
TOMM40
NCBI Official Synonym Symbols
TOM40; PEREC1; C19orf1; PER-EC1; D19S1177E
NCBI Protein Information
mitochondrial import receptor subunit TOM40 homolog; p38.5; protein Haymaker; mitochondrial outer membrane protein; translocase of outer membrane 40 kDa subunit homolog
UniProt Protein Name
Mitochondrial import receptor subunit TOM40 homolog
UniProt Gene Name
TOMM40
UniProt Synonym Gene Names
C19orf1; PEREC1; TOM40
UniProt Entry Name
TOM40_HUMAN
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Product Notes
The TOMM40 tomm40 (Catalog #AAA10745) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TOMM40 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF/ICC: 1:50 - 1:200. Researchers should empirically determine the suitability of the TOMM40 tomm40 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TOMM40, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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