IF (Immunofluorescence)
(Confocal immunofluorescent analysis of TOP1 Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Rabbit TOP1 Polyclonal Antibody | anti-TOP1 antibody
TOP1 Antibody (N-term)
Gene Names
TOP1; TOPI
Reactivity
Human (Predicted Reactivity: Hamster, Mouse, Rat)
Applications
Immunofluorescence, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
TOP1, Antibody; TOP1 Antibody (N-term); DNA topoisomerase 1; DNA topoisomerase I; TOP1; anti-TOP1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Hamster, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This TOP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-31 amino acids from the N-terminal region of human TOP1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
2-31
Sequence Length
765
Applicable Applications for anti-TOP1 antibody
IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of TOP1 Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of TOP1 Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
WB (Western Blot)
(TOP1 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the TOP1 antibody detected the TOP1 protein (arrow).)
WB (Western Blot)
(TOP1 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the TOP1 antibody detected the TOP1 protein (arrow).)
Related Product Information for anti-TOP1 antibody
This gene encodes a DNA topoisomerase, an enzyme that
controls and alters the topologic states of DNA during
transcription. This enzyme catalyzes the transient breaking and
rejoining of a single strand of DNA which allows the strands to
pass through one another, thus altering the topology of DNA. This
gene is localized to chromosome 20 and has pseudogenes which reside
on chromosomes 1 and 22.
controls and alters the topologic states of DNA during
transcription. This enzyme catalyzes the transient breaking and
rejoining of a single strand of DNA which allows the strands to
pass through one another, thus altering the topology of DNA. This
gene is localized to chromosome 20 and has pseudogenes which reside
on chromosomes 1 and 22.
Product Categories/Family for anti-TOP1 antibody
References
Kjeldsen, E., et al. Anticancer Res. 30(9):3257-3265(2010)
Reinhold, W.C., et al. Cancer Res. 70(6):2191-2203(2010)
Tesauro, C., et al. Biochem. J. 425(3):531-539(2010)
Ballot, C., et al. Mol. Cancer Ther. 8(12):3307-3317(2009)
Lebedeva, N.A., et al. Biochemistry Mosc. 74(11):1278-1284(2009)
Reinhold, W.C., et al. Cancer Res. 70(6):2191-2203(2010)
Tesauro, C., et al. Biochem. J. 425(3):531-539(2010)
Ballot, C., et al. Mol. Cancer Ther. 8(12):3307-3317(2009)
Lebedeva, N.A., et al. Biochemistry Mosc. 74(11):1278-1284(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
90726
NCBI Official Full Name
DNA topoisomerase 1
NCBI Official Synonym Full Names
topoisomerase (DNA) I
NCBI Official Symbol
TOP1
NCBI Official Synonym Symbols
TOPI
NCBI Protein Information
DNA topoisomerase 1
UniProt Protein Name
DNA topoisomerase 1
UniProt Gene Name
TOP1
UniProt Entry Name
TOP1_HUMAN
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Product Notes
The TOP1 top1 (Catalog #AAA287962) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 2-31. The TOP1 Antibody (N-term) reacts with Human (Predicted Reactivity: Hamster, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's TOP1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the TOP1 top1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TOP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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