FCM/FACS (Flow Cytometry)
(TPI1 Antibody (C-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit TPI1 Polyclonal Antibody | anti-TPI1 antibody
TPI1 Antibody (C-term)
Gene Names
TPI1; TIM; TPI; TPID; HEL-S-49
Reactivity
Human, Mouse, Rat (Predicted Reactivity: Bovine, Monkey, Rabbit)
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry
Synonyms
TPI1, Antibody; TPI1 Antibody (C-term); Triosephosphate isomerase; TIM; Triose-phosphate isomerase; TPI1; TPI; anti-TPI1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat (Predicted Reactivity: Bovine, Monkey, Rabbit)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This TPI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 258-286 amino acids from the C-terminal region of human TPI1.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Positions
258-286
Sequence Length
167
Applicable Applications for anti-TPI1 antibody
ELISA, WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry)
Cellular Location
Cytoplasm {ECO:0000255|PROSITE-ProRule:PRU10127}.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(TPI1 Antibody (C-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(TPI1 Antibody (C-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with TPI1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with TPI1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of lysates from A431, HepG2, LNCaP cell line and mouse testis, rat testis tissue lysates (from left to right), using TPI1 Antibody (C-term). AAA287424 was diluted at 1:1000 at each lane.A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from A431, HepG2, LNCaP cell line and mouse testis, rat testis tissue lysates (from left to right), using TPI1 Antibody (C-term). AAA287424 was diluted at 1:1000 at each lane.A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
Related Product Information for anti-TPI1 antibody
Function: Triosephosphate isomerase is an extremely efficient metabolic enzyme that catalyzes the interconversion between dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (G3P) in glycolysis and gluconeogenesis.
Background: TPI1 is an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis.
Background: TPI1 is an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis.
Product Categories/Family for anti-TPI1 antibody
References
Martins-de-Souza,D., et.al., BMC Psychiatry 9, 17 (2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
triosephosphate isomerase isoform 1
NCBI Official Synonym Full Names
triosephosphate isomerase 1
NCBI Official Symbol
TPI1
NCBI Official Synonym Symbols
TIM; TPI; TPID; HEL-S-49
NCBI Protein Information
triosephosphate isomerase
UniProt Protein Name
Triosephosphate isomerase
UniProt Gene Name
TPI1
UniProt Synonym Gene Names
TPI; TIM
UniProt Entry Name
TPIS_HUMAN
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Product Notes
The TPI1 tpi1 (Catalog #AAA287424) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 258-286. The TPI1 Antibody (C-term) reacts with Human, Mouse, Rat (Predicted Reactivity: Bovine, Monkey, Rabbit) and may cross-react with other species as described in the data sheet. AAA Biotech's TPI1 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the TPI1 tpi1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TPI1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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