IF (Immunofluorescence)
(Immunofluorescence staining of TPM1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human TPM1 polyclonal antibody (dilution ratio 1:100) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
Rabbit anti-Human TPM1 Polyclonal Antibody | anti-TPM1 antibody
Anti-TPM1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
TPM1, Antibody; Anti-TPM1 Antibody, Rabbit Polyclonal; tropomyosin 1 (alpha); Anti-C15orf13 Antibody; Anti-CMD1Y Antibody; Anti-CMH3 Antibody; Anti-HTM-alpha Antibody; Anti-LVNC9 Antibody; Anti-TMSA Antibody; anti-TPM1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human TPM1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-TPM1 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry)
Immunogen
E coli-derived Human TPM1 fragment
Preparation
Produced in rabbits immunized with E coli-derived Human TPM1 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of TPM1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human TPM1 polyclonal antibody (dilution ratio 1:100) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
IF (Immunofluorescence)
(Immunofluorescence staining of TPM1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human TPM1 polyclonal antibody (dilution ratio 1:100) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
IHC (Immunohistochemistry)
(Immunochemical staining of human TPM1 in human muscle with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of human TPM1 in human muscle with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-TPM1 antibody
TPM1, also known as tropomyosin-1, is a member of the tropomyosin family. Members of this family are highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. TPM1 is one type of alpha-helical chain that forms the predominant tropomyosin of striated muscle. It binds to actin filaments in muscle and non-muscle cells. TPM1 plays a central role, in association with the troponin complex, in the calcium-dependent regulation of vertebrate striated muscle contraction.
References
Mogensen J. et al., 1999, Cytogenet Cell Genet. 84 (1-2): 35-6.
Brown H R. et al., 1985, Proc Natl Acad Sci. 82 (8): 2359-63.
Lees-Miller JP. et al., 1992, BioEssays. 13 (9): 429-37.
Brown H R. et al., 1985, Proc Natl Acad Sci. 82 (8): 2359-63.
Lees-Miller JP. et al., 1992, BioEssays. 13 (9): 429-37.
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Product Notes
The TPM1 (Catalog #AAA258960) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TPM1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TPM1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the TPM1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TPM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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