WB (Western Blot)
(Detection of human and mouse TPRBK by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TPRBK antibody AAA212828 (lot AAA212828-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human, Mouse TPRBK Polyclonal Antibody | anti-TPRBK antibody
Rabbit anti-TPRBK Antibody, Affinity Purified
Gene Names
TTC28; TPRBK
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
TPRBK, Antibody; Rabbit anti-TPRBK Antibody, Affinity Purified; TPR repeat protein 28; TPR repeat-containing big gene cloned at Keio; TPRBK; TPR-containing big gene cloned at Keio; tetratricopeptide repeat protein 28; Tetratricopeptide repeat protein 28; tetratricopeptide repeat domain 28; anti-TPRBK antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-TPRBK antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 2431 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse TPRBK by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TPRBK antibody AAA212828 (lot AAA212828-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human and mouse TPRBK by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TPRBK antibody AAA212828 (lot AAA212828-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human TPRBK by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TPRBK antibody AAA212828 (lot AAA212828-1) used for IP at 6 ug per reaction. TPRBK was also immunoprecipitated by rabbit anti-TPRBK antibody BL17736. For blotting immunoprecipitated TPRBK, AAA212828 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human TPRBK by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TPRBK antibody AAA212828 (lot AAA212828-1) used for IP at 6 ug per reaction. TPRBK was also immunoprecipitated by rabbit anti-TPRBK antibody BL17736. For blotting immunoprecipitated TPRBK, AAA212828 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-TPRBK antibody
During mitosis, tetratricopeptide repeat protein 28 (TPRBK) may be involved in the condensation of spindle midzone microtubules, leading to the formation of midbody [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96AY4].
Product Categories/Family for anti-TPRBK antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
270,884 Da
NCBI Official Full Name
tetratricopeptide repeat protein 28
NCBI Official Synonym Full Names
tetratricopeptide repeat domain 28
NCBI Official Symbol
TTC28
NCBI Official Synonym Symbols
TPRBK
NCBI Protein Information
tetratricopeptide repeat protein 28; TPR repeat protein 28; TPR-containing big gene cloned at Keio; TPR repeat-containing big gene cloned at Keio
UniProt Protein Name
Tetratricopeptide repeat protein 28
UniProt Gene Name
TTC28
UniProt Synonym Gene Names
KIAA1043; TPRBK; TPR repeat protein 28
UniProt Entry Name
TTC28_HUMAN
Similar Products
Product Notes
The TPRBK ttc28 (Catalog #AAA212828) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-TPRBK Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TPRBK can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the TPRBK ttc28 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TPRBK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.