IF (Immunofluorescence)
(AAA328980 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328980 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Rabbit Transferrin Polyclonal Antibody | anti-TF antibody
Transferrin Antibody
Gene Names
TF; TFQTL1; PRO1557; PRO2086; HEL-S-71p
Reactivity
HumanPredicted Reactivity: Rabbit(86%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
Transferrin, Antibody; Transferrin Antibody; Apotransferrin; Beta 1 metal binding globulin; Beta-1 metal-binding globulin; DKFZp781D0156; PRO1400; PRO1557; PRO2086; Serotransferrin; Serotransferrin precursor; Siderophilin; TF; TFQTL1; Transferin; Transferrin; TRFE_HUMAN; anti-TF antibody
Host
Rabbit
Reactivity
Human
Predicted Reactivity: Rabbit(86%)
Predicted Reactivity: Rabbit(86%)
Clonality
Polyclonal
Isotype
IgG
Specificity
Transferrin Antibody detects endogenous levels of total Transferrin.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
698
Applicable Applications for anti-TF antibody
ELISA
Immunogen
A synthesized peptide derived from human Transferrin, corresponding to a region within the internal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA328980 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328980 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
IF (Immunofluorescence)
(AAA328980 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328980 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells, using Transferrin Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells, using Transferrin Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-TF antibody
Function: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
Subcellular Location: Secreted.
Tissue Specificity: Expressed by the liver and secreted in plasma.
Subunit Structure: Monomer.
Similarity: Belongs to the transferrin family.
Subcellular Location: Secreted.
Tissue Specificity: Expressed by the liver and secreted in plasma.
Subunit Structure: Monomer.
Similarity: Belongs to the transferrin family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)77kD.
Predicted Molecular Weight: (Calculated)77kDa.
Predicted Molecular Weight: (Calculated)77kDa.
NCBI Official Full Name
transferrin
NCBI Official Synonym Full Names
transferrin
NCBI Official Symbol
TF
NCBI Official Synonym Symbols
TFQTL1; PRO1557; PRO2086; HEL-S-71p
NCBI Protein Information
serotransferrin
UniProt Protein Name
Serotransferrin
UniProt Gene Name
TF
UniProt Synonym Gene Names
Transferrin
UniProt Entry Name
TRFE_HUMAN
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Product Notes
The TF tf (Catalog #AAA328980) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Transferrin Antibody reacts with Human Predicted Reactivity: Rabbit(86%) and may cross-react with other species as described in the data sheet. AAA Biotech's Transferrin can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the TF tf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Transferrin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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