WB (Western Blot)
(Detection of human TRAP100/MED24 by western blot. Samples: Whole cell lysate (50 ug) from RKO, GaMG, HEK293T, LNCaP, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRAP100/MED24 antibody (AAA211493 lot 2) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human, Mouse TRAP100/MED24 Polyclonal Antibody | anti-TRAP100/MED24 antibody
Rabbit anti-TRAP100/MED24 Antibody, Affinity Purified
Gene Names
MED24; CRSP4; ARC100; THRAP4; CRSP100; DRIP100; TRAP100
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
TRAP100/MED24, Antibody; Rabbit anti-TRAP100/MED24 Antibody, Affinity Purified; TRAP100; MED5; vitamin D3 receptor-interacting protein complex 100 kDa component; thyroid hormone receptor-associated protein complex 100 kDa component; thyroid hormone receptor-associated protein 4; THRAP4; mediator of RNA polymerase II transcription, subunit 24 homolog; mediator of RNA polymerase II transcription subunit 24; Mediator complex subunit 24; activator-recruited cofactor 100 kDa component; hTRAP100; ARC100; cofactor required for Sp1 transcriptional activation subunit 4; cofactor required for Sp1 transcriptional activation, subunit 4, 100kDa; CRSP complex subunit 4; CRSP100; CRSP4; DRIP100; vitamin D3 receptor-interacting protein complex component DRIP100; Mediator of RNA polymerase II transcription subunit 24; mediator complex subunit 24; anti-TRAP100/MED24 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-TRAP100/MED24 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, Chicken
Immunogen
Between 939 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human TRAP100/MED24 by western blot. Samples: Whole cell lysate (50 ug) from RKO, GaMG, HEK293T, LNCaP, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRAP100/MED24 antibody (AAA211493 lot 2) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human TRAP100/MED24 by western blot. Samples: Whole cell lysate (50 ug) from RKO, GaMG, HEK293T, LNCaP, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRAP100/MED24 antibody (AAA211493 lot 2) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of mouse TRAP100/MED24 by western blot. Samples: Whole cell lysate (25 ug) from NIH 3T3, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRAP100/MED24 antibody (AAA211493 lot 2) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of mouse TRAP100/MED24 by western blot. Samples: Whole cell lysate (25 ug) from NIH 3T3, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRAP100/MED24 antibody (AAA211493 lot 2) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human TRAP100/MED24 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRAP100/MED24 antibody (AAA211493 lot 2) used for IP at 6 ug per reaction. TRAP100/MED24 was also immunoprecipitated by a previous lot of this antibody (AAA211493 lot 1). For blotting immunoprecipitated TRAP100/MED24, AAA211493 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human TRAP100/MED24 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRAP100/MED24 antibody (AAA211493 lot 2) used for IP at 6 ug per reaction. TRAP100/MED24 was also immunoprecipitated by a previous lot of this antibody (AAA211493 lot 1). For blotting immunoprecipitated TRAP100/MED24, AAA211493 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-TRAP100/MED24 antibody
TRAP100/MED24 is a component of the multisubunit mediator complex that functions as an interface between gene-specific transcription factors and the RNA Pol II/general transcription factor machinery. The mediator complex was first identified as a coactivator of the thyroid hormone receptor and has since been observed to function as a coactivator of a variety of transcription factors. TRAP100/MED24 associates with mediator subunit TRAP220. Both are essential to mouse development and play a role in androgen receptor-mediated transcription. Studies in zebrafish have shown that TRAP100/MED24 is also important to retinal development.
Product Categories/Family for anti-TRAP100/MED24 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
108,938 Da
NCBI Official Full Name
mediator of RNA polymerase II transcription subunit 24 isoform 1
NCBI Official Synonym Full Names
mediator complex subunit 24
NCBI Official Symbol
MED24
NCBI Official Synonym Symbols
CRSP4; ARC100; THRAP4; CRSP100; DRIP100; TRAP100
NCBI Protein Information
mediator of RNA polymerase II transcription subunit 24; CRSP complex subunit 4; thyroid hormone receptor associated protein 4; thyroid hormone receptor-associated protein 4; activator-recruited cofactor 100 kDa component; cofactor required for Sp1 transcr
UniProt Protein Name
Mediator of RNA polymerase II transcription subunit 24
UniProt Gene Name
MED24
UniProt Synonym Gene Names
ARC100; CRSP4; DRIP100; KIAA0130; THRAP4; TRAP100; ARC100; CRSP complex subunit 4; Trap100; hTRAP100; DRIP100
UniProt Entry Name
MED24_HUMAN
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Product Notes
The TRAP100/MED24 med24 (Catalog #AAA211493) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-TRAP100/MED24 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TRAP100/MED24 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the TRAP100/MED24 med24 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRAP100/MED24, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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