IF (Immunofluorescence)
(Figure 2. IF analysis of TRF1/TERF1 using anti-TRF1/TERF1 antibody (AAA126231).TRF1/TERF1 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-TRF1/TERF1 Antibody (AAA126231) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
Rabbit anti-Human TRF1/TERF1 Polyclonal Antibody | anti-TERF1 antibody
Anti-TRF1/TERF1 Antibody Picoband
Gene Names
TERF1; TRF; PIN2; TRF1; TRBF1; t-TRF1; hTRF1-AS
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, ELISA
Purity
Immunogen affinity purified.
Synonyms
TRF1/TERF1, Antibody; Anti-TRF1/TERF1 Antibody Picoband; anti-TERF1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (varies by lot)
Applicable Applications for anti-TERF1 antibody
WB (Western Blot), ICC (Immunocytochemistry), IF (Immunofluorescence), ELISA
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Immunogen
E Coli-derived human TRF1/TERF1 recombinant protein (Position: Q119-R341).
Preparation and Storage
Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.
IF (Immunofluorescence)
(Figure 2. IF analysis of TRF1/TERF1 using anti-TRF1/TERF1 antibody (AAA126231).TRF1/TERF1 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-TRF1/TERF1 Antibody (AAA126231) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
IF (Immunofluorescence)
(Figure 2. IF analysis of TRF1/TERF1 using anti-TRF1/TERF1 antibody (AAA126231).TRF1/TERF1 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-TRF1/TERF1 Antibody (AAA126231) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
WB (Western Blot)
(Figure 1. Western blot analysis of TRF1/TERF1 using anti-TRF1/TERF1 antibody (AAA126231).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRF1/TERF1 antigen affinity purified polyclonal antibody (#AAA126231) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TRF1/TERF1 at approximately 55 kDa. The expected band size for TRF1/TERF1 is at 50 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of TRF1/TERF1 using anti-TRF1/TERF1 antibody (AAA126231).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRF1/TERF1 antigen affinity purified polyclonal antibody (#AAA126231) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TRF1/TERF1 at approximately 55 kDa. The expected band size for TRF1/TERF1 is at 50 kDa.)
Related Product Information for anti-TERF1 antibody
Telomeric repeat-binding factor 1 is a protein that in humans is encoded by the TERF1 gene. This gene encodes a telomere specific protein which is a component of the telomere nucleoprotein complex. This protein is present at telomeres throughout the cell cycle and functions as an inhibitor of telomerase, acting in cis to limit the elongation of individual chromosome ends. The protein structure contains a C-terminal Myb motif, a dimerization domain near its N-terminus and an acidic N-terminus. Two transcripts of this gene are alternatively spliced products.
Product Categories/Family for anti-TERF1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,246 Da
NCBI Official Full Name
telomeric repeat-binding factor 1 isoform 2
NCBI Official Synonym Full Names
telomeric repeat binding factor (NIMA-interacting) 1
NCBI Official Symbol
TERF1
NCBI Official Synonym Symbols
TRF; PIN2; TRF1; TRBF1; t-TRF1; hTRF1-AS
NCBI Protein Information
telomeric repeat-binding factor 1; NIMA-interacting protein 2; telomeric protein Pin2/TRF1; TTAGGG repeat-binding factor 1
UniProt Protein Name
Telomeric repeat-binding factor 1
UniProt Gene Name
TERF1
UniProt Synonym Gene Names
PIN2; TRBF1; TRF; TRF1
UniProt Entry Name
TERF1_HUMAN
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Product Notes
The TERF1 terf1 (Catalog #AAA126231) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TRF1/TERF1 Antibody Picoband reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRF1/TERF1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ICC (Immunocytochemistry), IF (Immunofluorescence), ELISA. Researchers should empirically determine the suitability of the TERF1 terf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRF1/TERF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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