WB (Western Blot)
(Detection of human and mouse Triad1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Triad1 antibody AAA212065 (lot AAA212065-3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human, Mouse Triad1 Polyclonal Antibody | anti-Triad1 antibody
Rabbit anti-Triad1 Antibody, Affinity Purified
Gene Names
ARIH2; ARI2; TRIAD1
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Triad1, Antibody; Rabbit anti-Triad1 Antibody, Affinity Purified; TRIAD1; Triad1 protein; RING-type E3 ubiquitin transferase ARIH2; protein ariadne-2 homolog; ariadne homolog 2; ARI-2; ARI2; all-trans retinoic acid inducible RING finger; E3 ubiquitin-protein ligase ARIH2; ariadne RBR E3 ubiquitin protein ligase 2; anti-Triad1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-Triad1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 443 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse Triad1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Triad1 antibody AAA212065 (lot AAA212065-3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human and mouse Triad1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Triad1 antibody AAA212065 (lot AAA212065-3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human Triad1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Triad1 antibody AAA212065 (lot AAA212065-3) used for IP at 3 ug per reaction. Triad1 was also immunoprecipitated by rabbit anti-Triad1 antibody For blotting immunoprecipitated Triad1, AAA212065 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human Triad1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Triad1 antibody AAA212065 (lot AAA212065-3) used for IP at 3 ug per reaction. Triad1 was also immunoprecipitated by rabbit anti-Triad1 antibody For blotting immunoprecipitated Triad1, AAA212065 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-Triad1 antibody
Triad1 is an E3 ubiquitin-protein ligase mediating 'Lys-48'-and 'Lys-63'-linked polyubiquitination and subsequent proteasomal degradation of modified proteins. Triad1 may play a role in myelopoiesis [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O95376].
Product Categories/Family for anti-Triad1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,819 Da
NCBI Official Full Name
Homo sapiens ariadne RBR E3 ubiquitin protein ligase 2 (ARIH2), mRNA
NCBI Official Synonym Full Names
ariadne RBR E3 ubiquitin protein ligase 2
NCBI Official Symbol
ARIH2
NCBI Official Synonym Symbols
ARI2; TRIAD1
NCBI Protein Information
E3 ubiquitin-protein ligase ARIH2; ARI-2; all-trans retinoic acid inducible RING finger; ariadne homolog 2; protein ariadne-2 homolog
UniProt Protein Name
E3 ubiquitin-protein ligase ARIH2
UniProt Gene Name
ARIH2
UniProt Synonym Gene Names
ARI2; TRIAD1; ARI-2; Protein ariadne-2 homolog
UniProt Entry Name
ARI2_HUMAN
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Product Notes
The Triad1 arih2 (Catalog #AAA212065) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Triad1 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Triad1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the Triad1 arih2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Triad1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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