IF (Immunofluorescence)
(Immunofluorescence staining of HepG2 cells with AAA234485 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Rabbit anti-Human TRIB1 Polyclonal Antibody | anti-TRIB1 antibody
TRIB1 Antibody
Gene Names
TRIB1; C8FW; GIG2; TRB1; GIG-2; SKIP1; TRB-1
Reactivity
Human
Applications
Immunofluorescence, Western Blot, ELISA
Purity
>95%, Protein G purified
Synonyms
TRIB1, Antibody; TRIB1 Antibody; Tribbles homolog 1; TRB-1; G-protein-coupled receptor-induced gene 2 protein; GIG-2; SKIP1; TRIB1; anti-TRIB1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
206
Applicable Applications for anti-TRIB1 antibody
IF (Immunofluorescence), WB (Western Blot), ELISA
Immunogen
Recombinant Human Tribbles homolog 1 protein (3-91AA)
Conjugation
Non-conjugated
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
IF (Immunofluorescence)
(Immunofluorescence staining of HepG2 cells with AAA234485 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of HepG2 cells with AAA234485 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
WB (Western Blot)
(Western BlotPositive WB detected in: Raji whole cell lysateAll lanes: TRIB1 antibody at 4.3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 42, 24 KDaObserved band size: 42 KDa)
WB (Western Blot)
(Western BlotPositive WB detected in: Raji whole cell lysateAll lanes: TRIB1 antibody at 4.3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 42, 24 KDaObserved band size: 42 KDa)
Related Product Information for anti-TRIB1 antibody
Adapter protein involved in protein degradation by interacting with RFWD2/COP1 ubiquitin ligase (PubMed:27041596). The RFWD2-binding motif is masked by autoinhibitory interactions with the protein kinase domain (PubMed:26455797). Serves to alter RFWD2 substrate specificity by directing the activity of RFWD2 toward CEBPA (PubMed:27041596). Binds selectively the recognition sequence of CEBPA (PubMed:26455797). Regulates myeloid cell differentiation by altering the expression of CEBPA in a RFWD2-dependent manner (By similarity). Controls macrophage, eosinophil and neutrophil differentiation via the COP1-binding domain (By similarity). Interacts with MAPK kinases and regulates activation of MAP kinases, but has no kinase activity (PubMed:15299019, PubMed:26455797).
Product Categories/Family for anti-TRIB1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,473 Da
NCBI Official Full Name
tribbles homolog 1 isoform 2
NCBI Official Synonym Full Names
tribbles pseudokinase 1
NCBI Official Symbol
TRIB1
NCBI Official Synonym Symbols
C8FW; GIG2; TRB1; GIG-2; SKIP1; TRB-1
NCBI Protein Information
tribbles homolog 1
UniProt Protein Name
Tribbles homolog 1
UniProt Gene Name
TRIB1
UniProt Synonym Gene Names
TRB-1; GIG-2
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Product Notes
The TRIB1 trib1 (Catalog #AAA234485) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRIB1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRIB1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the TRIB1 trib1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRIB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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