FCM/FACS (Flow Cytometry)
(TRIB2 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit TRIB2 Polyclonal Antibody | anti-TRIB2 antibody
TRIB2 Antibody (N-term)
Gene Names
TRIB2; C5FW; TRB2; GS3955
Reactivity
Human (Predicted Reactivity: Bovine, Mouse)
Applications
Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
TRIB2, Antibody; TRIB2 Antibody (N-term); Tribbles homolog 2; TRB-2; TRIB2 HGNC:30809); anti-TRIB2 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This TRIB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human TRIB2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
1-30
Sequence Length
343
Applicable Applications for anti-TRIB2 antibody
WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(TRIB2 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(TRIB2 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(TRIB2 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded mouse brain followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TRIB2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(TRIB2 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded mouse brain followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TRIB2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of TRIB2 (arrow) using rabbit polyclonal TRIB2 N-term. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TRIB2 gene (Lane 2).)
WB (Western Blot)
(Western blot analysis of TRIB2 (arrow) using rabbit polyclonal TRIB2 N-term. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TRIB2 gene (Lane 2).)
Related Product Information for anti-TRIB2 antibody
TRIB2 is one of three members of the Tribbles family. The Tribbles members share a Trb domain, which is homologous to protein serine-threonine kinases, but lacks the active site lysine and probably lacks a catalytic function. The Tribbles proteins interact and modulate the activity of signal transduction pathways in a number of physiological and pathological processes. This Tribbles member induces apoptosis of cells mainly of the hematopoietic origin. It has been identified as a protein up-regulated by inflammatory stimuli in myeloid (THP-1) cells, and also as an oncogene that inactivates the transcription factor C/EBPalpha (CCAAT/enhancer-binding protein alpha) and causes acute myelogenous leukemia.
Product Categories/Family for anti-TRIB2 antibody
References
Hegele,R.A., et.al., Hum. Mol. Genet. 18 (21), 4189-4194 (2009)
Eder,K., et.al., Int. Immunol. 20 (12), 1543-1550 (2008)
Eder,K., et.al., Int. Immunol. 20 (12), 1543-1550 (2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38801
NCBI Official Full Name
tribbles homolog 2
NCBI Official Synonym Full Names
tribbles pseudokinase 2
NCBI Official Symbol
TRIB2
NCBI Official Synonym Symbols
C5FW; TRB2; GS3955
NCBI Protein Information
tribbles homolog 2
UniProt Protein Name
Tribbles homolog 2
UniProt Gene Name
TRIB2
UniProt Synonym Gene Names
TRB-2
UniProt Entry Name
TRIB2_HUMAN
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Product Notes
The TRIB2 trib2 (Catalog #AAA290077) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-30. The TRIB2 Antibody (N-term) reacts with Human (Predicted Reactivity: Bovine, Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's TRIB2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the TRIB2 trib2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRIB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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