FCM/FACS (Flow Cytometry)
(Blank control (Black line): Mouse spleen(Black). Primary Antibody (green line): Rabbit Anti-TRIM32 antibody (bs-0964R) Dilution: 3 ug /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG .Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1 ug /test.Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 10,000 events was performed.)
Rabbit anti-Rat TRIM32 Polyclonal Antibody | anti-TRIM32 antibody
TRIM32 Polyclonal Antibody
Gene Names
Trim32; 3f3; BBS11; Zfp117; 1810045E12Rik
Reactivity
Rat
Applications
Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
TRIM32, Antibody; TRIM32 Polyclonal Antibody; E3 ubiquitin-protein ligase TRIM32; 2.3.2.27; RING-type E3 ubiquitin transferase TRIM32; Tripartite motif-containing protein 32; Trim32; anti-TRIM32 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Applicable Applications for anti-TRIM32 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Blank control (Black line): Mouse spleen(Black). Primary Antibody (green line): Rabbit Anti-TRIM32 antibody (bs-0964R) Dilution: 3 ug /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG .Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1 ug /test.Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 10,000 events was performed.)
FCM/FACS (Flow Cytometry)
(Blank control (Black line): Mouse spleen(Black). Primary Antibody (green line): Rabbit Anti-TRIM32 antibody (bs-0964R) Dilution: 3 ug /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG .Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1 ug /test.Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 10,000 events was performed.)
IHC (Immunohistochemistry)
(Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (TRIM32) Polyclonal Antibody, Unconjugated (bs-0964R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
IHC (Immunohistochemistry)
(Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (TRIM32) Polyclonal Antibody, Unconjugated (bs-0964R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
72,057 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase TRIM32
NCBI Official Synonym Full Names
tripartite motif-containing 32
NCBI Official Symbol
Trim32
NCBI Official Synonym Symbols
3f3; BBS11; Zfp117; 1810045E12Rik
NCBI Protein Information
E3 ubiquitin-protein ligase TRIM32
UniProt Protein Name
E3 ubiquitin-protein ligase TRIM32
UniProt Gene Name
Trim32
UniProt Entry Name
TRI32_MOUSE
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Product Notes
The TRIM32 trim32 (Catalog #AAA291272) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRIM32 Polyclonal Antibody reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TRIM32 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the TRIM32 trim32 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRIM32, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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