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product-image-AAA229063_IHC11.jpg IHC (Immunohistochemisry) (Immunofluorescence staining of Hela cells cells with AAA229063 at 1:133,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

Rabbit anti-Human TRIM35 Polyclonal Antibody | anti-TRIM35 antibody

TRIM35 Antibody

Gene Names
TRIM35; HLS5; MAIR
Reactivity
Human
Applications
ELISA
Purity
>95%,Protein G purified
Synonyms
TRIM35, Antibody; TRIM35 Antibody; Tripartite motif-containing protein 35; Hemopoietic lineage switch protein 5; TRIM35; HLS5; KIAA1098; anti-TRIM35 antibody
Ordering
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%,Protein G purified
Form/Format
Liquid
Sequence Length
493
Applicable Applications for anti-TRIM35 antibody
ELISA
Immunogen
Recombinant human Tripartite motif-containing protein 35 protein
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-79787 / sc-134121
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

IHC (Immunohistochemisry)

(Immunofluorescence staining of Hela cells cells with AAA229063 at 1:133,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

product-image-AAA229063_IHC11.jpg IHC (Immunohistochemisry) (Immunofluorescence staining of Hela cells cells with AAA229063 at 1:133,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

IHC (Immunohiostchemistry)

(IHC image of AAA229063 diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

product-image-AAA229063_IHC13.jpg IHC (Immunohiostchemistry) (IHC image of AAA229063 diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

IHC (Immunohistochemistry)

(IHC image of AAA229063 diluted at 1:400 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

product-image-AAA229063_IHC15.jpg IHC (Immunohistochemistry) (IHC image of AAA229063 diluted at 1:400 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Related Product Information for anti-TRIM35 antibody
May play a role as a tumor suppressor. Implicated in the cell death mechanism.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
55,914 Da
NCBI Official Full Name
tripartite motif-containing protein 35 isoform 1
NCBI Official Synonym Full Names
tripartite motif containing 35
NCBI Official Symbol
TRIM35
NCBI Official Synonym Symbols
HLS5; MAIR
NCBI Protein Information
tripartite motif-containing protein 35
UniProt Protein Name
Tripartite motif-containing protein 35
UniProt Gene Name
TRIM35
UniProt Synonym Gene Names
HLS5; KIAA1098
UniProt Entry Name
TRI35_HUMAN

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Product Notes

The TRIM35 trim35 (Catalog #AAA229063) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRIM35 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRIM35 can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the TRIM35 trim35 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRIM35, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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