IP (Immunoprecipitation)
(TNNC1 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate4 uL anti-TNNC1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-TNNC1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 18 kDaObserved band size :16 kDa)
Rabbit Troponin C Polyclonal Antibody | anti-TNNC1 antibody
Anti-Troponin C Antibody, Rabbit Polyclonal
Gene Names
TNNC1; TNC; TN-C; TNNC; CMD1Z; CMH13
Reactivity
Human, Cynomolgus
Applications
Immunoprecipitation, Immunohistochemistry, ELISA
Purity
Protein A & Antigen Affinity
Synonyms
Troponin C, Antibody; Anti-Troponin C Antibody, Rabbit Polyclonal; Troponin C/TNNC1 Antibody, Rabbit PAb, Antigen Affinity Purified; troponin C type 1 (slow); Anti-CMD1Z Antibody; Anti-CMH13 Antibody; Anti-TN-C Antibody; Anti-TNC Antibody; Anti-TNNC Antibody; anti-TNNC1 antibody
Host
Rabbit
Reactivity
Human, Cynomolgus
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human Troponin C
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-TNNC1 antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA
Immunogen
Recombinant Human Troponin C/TNNC1 Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human Troponin C/TNNC1 (rh Troponin C/TNNC1). Troponin C/TNNC1 specific IgG was purified by Human Troponin C/TNNC1 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(TNNC1 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate4 uL anti-TNNC1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-TNNC1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 18 kDaObserved band size :16 kDa)
IP (Immunoprecipitation)
(TNNC1 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate4 uL anti-TNNC1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-TNNC1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 18 kDaObserved band size :16 kDa)
IHC (Immunohiostchemistry)
(Immunochemical staining TNNC1 in cynomolgus heart with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining TNNC1 in cynomolgus heart with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining TNNC1 in human skeletal muscle with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining TNNC1 in human skeletal muscle with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-TNNC1 antibody
Troponin I, also known as TNNC1, is part of the troponin complex. This complex contains 3 subunits: troponin I (TnI), troponin T (TnT) and troponin C (TnC). Troponin I is the inhibitory subunit, blocking actin-myosin interactions and thereby mediating striated muscle relaxation. It binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place. Because of it myosin cannot bind actin in relaxed muscle. When calcium binds to the Troponin C it causes conformational changes which lead to dislocation of troponin I and finally tropomyosin leaves the binding site for myosin on actin leading to contraction of muscle.
References
Kalaji FR. et al., 2012, Saudi J Kidney Dis Transpl. 23 (5): 939-45.Wijnker PJ. et al., 2013, Am J Physiol Heart Circ Physiol. 304 (2): H260-8.Solaro RJ. et al., 2013, Circ Res. 112 (2): 355-66.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18,020 Dalton
NCBI Official Full Name
troponin C, slow skeletal and cardiac muscles
NCBI Official Synonym Full Names
troponin C type 1 (slow)
NCBI Official Symbol
TNNC1
NCBI Official Synonym Symbols
TNC; TN-C; TNNC; CMD1Z; CMH13
NCBI Protein Information
troponin C, slow skeletal and cardiac muscles; cardiac troponin C; slow twitch skeletal/cardiac muscle troponin C; troponin C1, slow
UniProt Protein Name
Troponin C, slow skeletal and cardiac muscles
UniProt Gene Name
TNNC1
UniProt Synonym Gene Names
TNNC; TN-C
UniProt Entry Name
TNNC1_HUMAN
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Product Notes
The TNNC1 tnnc1 (Catalog #AAA255287) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Troponin C Antibody, Rabbit Polyclonal reacts with Human, Cynomolgus and may cross-react with other species as described in the data sheet. AAA Biotech's Troponin C can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the TNNC1 tnnc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Troponin C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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