WB (Western Blot)
(CHAK1 Antibody (AAA28787) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the CHAK1 antibody detected the CHAK1 protein (arrow).)
Rabbit TRPM7 (CHAK1) Polyclonal Antibody | anti-TRPM7 antibody
TRPM7 (CHAK1) Antibody (C-term)
Gene Names
TRPM7; CHAK; CHAK1; ALSPDC; LTRPC7; LTrpC-7; TRP-PLIK
Reactivity
Human, MousePredicted: Rat
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
TRPM7 (CHAK1), Antibody; TRPM7 (CHAK1) Antibody (C-term); CHAK1, LTRPC7; anti-TRPM7 antibody
Host
Rabbit
Reactivity
Human, Mouse
Predicted: Rat
Predicted: Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
This TRPM7 (CHAK1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1821-1850 amino acids from the C-terminal region of human TRPM7 (CHAK1).
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Positions
1821-1850
Sequence Length
1,865
Applicable Applications for anti-TRPM7 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry-Parrafin (IHC-P)
Application Notes
WB~~1:1000
IHC-P~~1:50~100
IHC-P~~1:50~100
Function
Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. Involved in TNF- induced necroptosis downstream of MLKL by mediating calcium influx. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1).
Cellular Location
Membrane; Multi-pass membrane protein
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(CHAK1 Antibody (AAA28787) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the CHAK1 antibody detected the CHAK1 protein (arrow).)
WB (Western Blot)
(CHAK1 Antibody (AAA28787) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the CHAK1 antibody detected the CHAK1 protein (arrow).)
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody (AAA28787), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody (AAA28787), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
WB (Western Blot)
(Western blot analysis of anti-CHAK1 Antibody (AAA28787) (C-term) in mouse kidney tissue lysates (35ug/lane). CHAK1 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of anti-CHAK1 Antibody (AAA28787) (C-term) in mouse kidney tissue lysates (35ug/lane). CHAK1 (arrow) was detected using the purified Pab.)
Related Product Information for anti-TRPM7 antibody
TRPCs, mammalian homologs of the Drosophila transient receptor potential (trp) protein, are ion channels that are thought to mediate capacitative calcium entry into the cell. TRP-PLIK is a protein that is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it is capable of phosphorylating itself and other substrates. The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants.[supplied by OMIM]
Product Categories/Family for anti-TRPM7 antibody
References
Blume-Jensen P, et al. Nature 2001. 411: 355.
Cantrell D, J. Cell Sci. 2001. 114: 1439.
Jhiang S Oncogene 2000. 19: 5590.
Manning G, et al. Science 2002. 298: 1912.
Moller, D, et al. Am. J. Physiol. 1994. 266: C351-C359.
Robertson, S. et al. Trends Genet. 2000. 16: 368.
Robinson D, et al. Oncogene 2000. 19: 5548.
Van der Ven, P, et al. Hum. Molec. Genet. 1993. 2: 1889.
Vanhaesebroeck, B, et al. Biochem. J. 2000. 346: 561.
Van Weering D, et al. Recent Results Cancer Res. 1998. 154: 271.
Cantrell D, J. Cell Sci. 2001. 114: 1439.
Jhiang S Oncogene 2000. 19: 5590.
Manning G, et al. Science 2002. 298: 1912.
Moller, D, et al. Am. J. Physiol. 1994. 266: C351-C359.
Robertson, S. et al. Trends Genet. 2000. 16: 368.
Robinson D, et al. Oncogene 2000. 19: 5548.
Van der Ven, P, et al. Hum. Molec. Genet. 1993. 2: 1889.
Vanhaesebroeck, B, et al. Biochem. J. 2000. 346: 561.
Van Weering D, et al. Recent Results Cancer Res. 1998. 154: 271.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
transient receptor potential cation channel subfamily M member 7 isoform a
NCBI Official Synonym Full Names
transient receptor potential cation channel, subfamily M, member 7
NCBI Official Symbol
TRPM7
NCBI Official Synonym Symbols
CHAK; CHAK1; ALSPDC; LTRPC7; LTrpC-7; TRP-PLIK
NCBI Protein Information
transient receptor potential cation channel subfamily M member 7
UniProt Protein Name
Transient receptor potential cation channel subfamily M member 7
UniProt Gene Name
TRPM7
UniProt Synonym Gene Names
CHAK1; LTRPC7; LTrpC-7; LTrpC7
UniProt Entry Name
TRPM7_HUMAN
Similar Products
Product Notes
The TRPM7 trpm7 (Catalog #AAA28787) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1821-1850. The TRPM7 (CHAK1) Antibody (C-term) reacts with Human, Mouse Predicted: Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TRPM7 (CHAK1) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-Parrafin (IHC-P). WB~~1:1000 IHC-P~~1:50~100. Researchers should empirically determine the suitability of the TRPM7 trpm7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRPM7 (CHAK1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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