WB (Western Blot)
(Western blot analysis of TSEN2 expression in TSEN2 transfected 293T lysate, The lane on the left is treated with the antigen-specific peptide.)
Rabbit anti-Human TSEN2 Polyclonal Antibody | anti-TSEN2 antibody
TSEN2 Antibody
Gene Names
TSEN2; SEN2; PCH2B; SEN2L
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
TSEN2, Antibody; TSEN2 Antibody; HsSen2; MGC2776; MGC4440; SEN2; SEN2_HUMAN; SEN2L; tRNA intron endonuclease Sen2; tRNA splicing endonuclease 2 homolog (SEN2, S. cerevisiae); tRNA splicing endonuclease subunit Sen2; tRNA-intron endonuclease sen2; tRNA-splicing endonuclease subunit Sen2; TSEN 2; TSEN2; anti-TSEN2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
TSEN2 antibody detects endogenous levels of total TSEN2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
465
Applicable Applications for anti-TSEN2 antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide
Subcellular Location
Nucleus
Tissue Specificity
Isoform 1 and isoform 2 are widely expressed at very low level.
Conjugation
Unconjugated
WB (Western Blot)
(Western blot analysis of TSEN2 expression in TSEN2 transfected 293T lysate, The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of TSEN2 expression in TSEN2 transfected 293T lysate, The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry)
(AAA323354 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA323354 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-TSEN2 antibody
Description: Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'-and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2', 3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Isoform 1 probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events. Isoform 2 is responsible for processing a yet unknown RNA substrate. The complex containing isoform 2 is not able to cleave pre-tRNAs properly, although it retains endonucleolytic activity.
Subunit Structure: tRNA splicing endonuclease is a heterotetramer composed of isoform 1 of TSEN2, TSEN15, TSEN34/LENG5 and TSEN54. tRNA splicing endonuclease complex also contains proteins of the pre-mRNA 3'-end processing machinery such as CLP1, CPSF1, CPSF4 and CSTF2. Isoform 2 belongs to a different complex that contains TSEN54 but low level of TSEN15 and TSEN34/LENG5.
Similarity: Belongs to the tRNA-intron endonuclease family.
Subunit Structure: tRNA splicing endonuclease is a heterotetramer composed of isoform 1 of TSEN2, TSEN15, TSEN34/LENG5 and TSEN54. tRNA splicing endonuclease complex also contains proteins of the pre-mRNA 3'-end processing machinery such as CLP1, CPSF1, CPSF4 and CSTF2. Isoform 2 belongs to a different complex that contains TSEN54 but low level of TSEN15 and TSEN34/LENG5.
Similarity: Belongs to the tRNA-intron endonuclease family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 54 kDa
Predicted: 54 kDa
Predicted: 54 kDa
NCBI Official Full Name
tRNA-splicing endonuclease subunit Sen2 isoform 1
NCBI Official Synonym Full Names
tRNA splicing endonuclease subunit 2
NCBI Official Symbol
TSEN2
NCBI Official Synonym Symbols
SEN2; PCH2B; SEN2L
NCBI Protein Information
tRNA-splicing endonuclease subunit Sen2
UniProt Protein Name
tRNA-splicing endonuclease subunit Sen2
UniProt Gene Name
TSEN2
UniProt Synonym Gene Names
SEN2; HsSen2
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Product Notes
The TSEN2 tsen2 (Catalog #AAA323354) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TSEN2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TSEN2 can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the TSEN2 tsen2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TSEN2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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