WB (Western Blot)
(Western blot analysis of extracts from HT-29 cells, using TSSK4 antibody.)
Rabbit anti-Human, Mouse TSSK4 Polyclonal Antibody | anti-TSSK4 antibody
TSSK4 Antibody
Gene Names
TSSK4; TSK4; TSK-4; TSSK5; STK22E; C14orf20
Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
TSSK4, Antibody; TSSK4 Antibody; C14orf20; Serine/threonine-protein kinase 22E; STK22E; Testis-specific kinase 4; Testis-specific serine/threonine-protein kinase 4; TSK-4; TSSK-4; Tssk4; TSSK4_HUMAN; TSSK5; anti-TSSK4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
TSSK4 antibody detects endogenous levels of total TSSK4
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
338
Applicable Applications for anti-TSSK4 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide
Tissue Specificity
Expressed only in the testis.
Predicted Cross Reactivity
Pig, Horse, Sheep, Rabbit, Dog
Similarity
Pig (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from HT-29 cells, using TSSK4 antibody.)
WB (Western Blot)
(Western blot analysis of extracts from HT-29 cells, using TSSK4 antibody.)
IF (Immunofluorescence)
(AAA325207 staining 293T by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IF (Immunofluorescence)
(AAA325207 staining 293T by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-TSSK4 antibody
Function: May be involved in a signaling pathway during male germ cell development or mature sperm function (By similarity). Phosphorylates CREB1 on Ser-133 and stimulates downstream signaling.
Post-translational Modifications: Autophosphorylated.
Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Post-translational Modifications: Autophosphorylated.
Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 40 kD
Predicted: 38 kDa
Predicted: 38 kDa
NCBI Official Full Name
testis-specific serine/threonine-protein kinase 4 isoform 1
NCBI Official Synonym Full Names
testis specific serine kinase 4
NCBI Official Symbol
TSSK4
NCBI Official Synonym Symbols
TSK4; TSK-4; TSSK5; STK22E; C14orf20
NCBI Protein Information
testis-specific serine/threonine-protein kinase 4
UniProt Protein Name
Testis-specific serine/threonine-protein kinase 4
UniProt Gene Name
TSSK4
UniProt Synonym Gene Names
C14orf20Imported; TSK-4Curated; TSSK-4ImportedManual assertion inferred from database entriesiHGNC:19825; Testis-specific kinase 4Curated
Similar Products
Product Notes
The TSSK4 tssk4 (Catalog #AAA325207) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TSSK4 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TSSK4 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the TSSK4 tssk4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TSSK4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.