KO (Knockout Validation)
(Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: TTK knockout Hela cell lysate; Predicted MW: 97kd Observed MW: 100kd Primary Ab: 5ug/ml Rabbit Anti-Human TTK Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)
Rabbit anti-Human, Mouse TTK Protein Kinase Polyclonal Antibody | anti-TTK antibody
Polyclonal Antibody to TTK Protein Kinase (TTK)
Gene Names
TTK; ESK; PYT; CT96; MPH1; MPS1; MPS1L1
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
TTK Protein Kinase, Antibody; Polyclonal Antibody to TTK Protein Kinase (TTK); anti-TTK antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against TTK. It has beenselected for its ability to recognize TTK in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
0.99mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GSGGSSKVF QVLNEKKQIY AIKYVNLEEA DNQTLDSYRN EIAYLNKLQQ HSDKIIRLYD YEITDQYIYM VMECGNIDLN SWLKKKKSID PWERKSYWKN MLEAVHTIHQ HGIVHSDLKP ANFLIVDGML KLIDFGIANQ MQPDTTSVVK DSQVGTVNYM PPEAIKDMSS SRENGKSKSK ISPKSDVWSL GCILYYMTYG
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GSGGSSKVF QVLNEKKQIY AIKYVNLEEA DNQTLDSYRN EIAYLNKLQQ HSDKIIRLYD YEITDQYIYM VMECGNIDLN SWLKKKKSID PWERKSYWKN MLEAVHTIHQ HGIVHSDLKP ANFLIVDGML KLIDFGIANQ MQPDTTSVVK DSQVGTVNYM PPEAIKDMSS SRENGKSKSK ISPKSDVWSL GCILYYMTYG
Sequence Length
882
Applicable Applications for anti-TTK antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 1-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Fragment
TTK (Gly532~Leu786)
Organism Species
Homo sapiens (Human)
Cross Reactivity
Human, Mouse
Conjugate
No Conjugate
Immunogen
Recombinant TTK (Gly532~Leu786) expressed in E Coli.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
KO (Knockout Validation)
(Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: TTK knockout Hela cell lysate; Predicted MW: 97kd Observed MW: 100kd Primary Ab: 5ug/ml Rabbit Anti-Human TTK Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)
KO (Knockout Validation)
(Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: TTK knockout Hela cell lysate; Predicted MW: 97kd Observed MW: 100kd Primary Ab: 5ug/ml Rabbit Anti-Human TTK Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)
WB (Western Blot)
(Western Blot: Sample: Human Hela Cells.)
WB (Western Blot)
(Western Blot: Sample: Human Hela Cells.)
Product Categories/Family for anti-TTK antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
96,944 Da
NCBI Official Full Name
dual specificity protein kinase TTK isoform 2
NCBI Official Synonym Full Names
TTK protein kinase
NCBI Official Symbol
TTK
NCBI Official Synonym Symbols
ESK; PYT; CT96; MPH1; MPS1; MPS1L1
NCBI Protein Information
dual specificity protein kinase TTK
UniProt Protein Name
Dual specificity protein kinase TTK
UniProt Gene Name
TTK
UniProt Synonym Gene Names
MPS1; MPS1L1; PYT
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Product Notes
The TTK ttk (Catalog #AAA20893) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to TTK Protein Kinase (TTK) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TTK Protein Kinase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 1-5ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the TTK ttk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GSGGSSKVF QVLNEKKQIY AIKYVNLEEA DNQTLDSYRN EIAYLNKLQQ HSDKIIRLYD YEITDQYIYM VMECGNIDLN SWLKKKKSID PWERKSYWKN MLEAVHTIHQ HGIVHSDLKP ANFLIVDGML KLIDFGIANQ MQPDTTSVVK DSQVGTVNYM PPEAIKDMSS SRENGKSKSK ISPKSDVWSL GCILYYMTYG. It is sometimes possible for the material contained within the vial of "TTK Protein Kinase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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