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Knockout Validation
(Knockout Validation: Lane 1: Wild-type K562 cell lysate; Lane 2: TNFRSF10B knockout K562 cell lysate; Predicted MW: 48,45,13kDa Observed MW: 40kDa Primary Ab: 1ug/ml Rabbit Anti-Human TNFRSF10B Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Rabbit anti-Human Tumor Necrosis Factor Receptor Superfamily Polyclonal Antibody | anti-TNFRSF10B antibody
Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 10B (TNFRSF10B)
Gene Names
TNFRSF10B; DR5; CD262; KILLER; TRICK2; TRICKB; ZTNFR9; TRAILR2; TRICK2A; TRICK2B; TRAIL-R2; KILLER/DR5
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Affinity Chromatography
Synonyms
Tumor Necrosis Factor Receptor Superfamily, Antibody; Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 10B (TNFRSF10B); anti-TNFRSF10B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against TNFRSF10B. It has beenselected for its ability to recognize TNFRSF10B in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and GST-tag and its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-SLLWKKV LPYLKGICSG GGGDPERVDR SSQRPGAEDN VLNEIVSILQ PTQVPEQEME VQEPAEPTGV NMLSPGESEH LLEPAEAERS QRRRLLVPAN EGDPTETLRQ CFDDFADLVP FDSWEPLMRK LGLMDNEIKV AKAEAAGHRD TLYTMLIKWV NKTGRDASVH TLLDALETLG ERLAKQKIED
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-SLLWKKV LPYLKGICSG GGGDPERVDR SSQRPGAEDN VLNEIVSILQ PTQVPEQEME VQEPAEPTGV NMLSPGESEH LLEPAEAERS QRRRLLVPAN EGDPTETLRQ CFDDFADLVP FDSWEPLMRK LGLMDNEIKV AKAEAAGHRD TLYTMLIKWV NKTGRDASVH TLLDALETLG ERLAKQKIED
Applicable Applications for anti-TNFRSF10B antibody
ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot)
Fragment
TNFRSF10B (Ser234~Ala435)
Organism Species
Homo sapiens (Human)
Cross Reactivity
Human
Conjugate
No Conjugate
Immunogen
Recombinant TNFRSF10B (Ser234~Ala435) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Knockout Validation
(Knockout Validation: Lane 1: Wild-type K562 cell lysate; Lane 2: TNFRSF10B knockout K562 cell lysate; Predicted MW: 48,45,13kDa Observed MW: 40kDa Primary Ab: 1ug/ml Rabbit Anti-Human TNFRSF10B Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Knockout Validation
(Knockout Validation: Lane 1: Wild-type K562 cell lysate; Lane 2: TNFRSF10B knockout K562 cell lysate; Predicted MW: 48,45,13kDa Observed MW: 40kDa Primary Ab: 1ug/ml Rabbit Anti-Human TNFRSF10B Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemisry)
(DAB staining on fromalin fixed paraffin-embedded Ovary tissue))
IHC (Immunohistochemisry)
(DAB staining on fromalin fixed paraffin-embedded Ovary tissue))
WB (Western Blot)
(Western Blot: Sample: Recombinant TNFRSF10B, Human.)
WB (Western Blot)
(Western Blot: Sample: Recombinant TNFRSF10B, Human.)
WB (Western Blot)
(Western Blot: Sample: Lane1: Human K562 Cells; Lane2: Human Lung Tissue.)
WB (Western Blot)
(Western Blot: Sample: Lane1: Human K562 Cells; Lane2: Human Lung Tissue.)
Product Categories/Family for anti-TNFRSF10B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,083 Da
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 10B isoform 1
NCBI Official Synonym Full Names
TNF receptor superfamily member 10b
NCBI Official Symbol
TNFRSF10B
NCBI Official Synonym Symbols
DR5; CD262; KILLER; TRICK2; TRICKB; ZTNFR9; TRAILR2; TRICK2A; TRICK2B; TRAIL-R2; KILLER/DR5
NCBI Protein Information
tumor necrosis factor receptor superfamily member 10B
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 10B
UniProt Gene Name
TNFRSF10B
UniProt Synonym Gene Names
DR5; KILLER; TRAILR2; TRICK2; ZTNFR9; TRAIL receptor 2; TRAIL-R2
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Product Notes
The TNFRSF10B tnfrsf10b (Catalog #AAA143142) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 10B (TNFRSF10B) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Tumor Necrosis Factor Receptor Superfamily can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the TNFRSF10B tnfrsf10b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and GST-tag and its sequence is listed below. MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-SLL WKKV LPYLKGICSG GGGDPERVDR SSQRPGAEDN VLNEIVSILQ PTQVPEQEME VQEPAEPTGV NMLSPGESEH LLEPAEAERS QRRRLLVPAN EGDPTETLRQ CFDDFADLVP FDSWEPLMRK LGLMDNEIKV AKAEAAGHRD TLYTMLIKWV NKTGRDASVH TLLDALETLG ERLAKQKIED. It is sometimes possible for the material contained within the vial of "Tumor Necrosis Factor Receptor Superfamily, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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