Rabbit anti-Human, Rat TXK Polyclonal Antibody | anti-TXK antibody

TXK Antibody

Cat. Num. AAA233307

• Gene Names: TXK; RLK; TKL; BTKL; PTK4; PSCTK5
• Reactivity: Human, Rat
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
• Purity: >95%, Protein G purified
• Synonyms: TXK, Antibody; TXK Antibody; Tyrosine-protein kinase TXK (EC 2.7.10.2) (Protein-tyrosine kinase 4) (Resting lymphocyte kinase), TXK, PTK4 RLK; anti-TXK antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid

• Applicable Applications for anti-TXK antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Immunogen: Recombinant Human Tyrosine-protein kinase TXK protein (32-83AA)
• Conjugation: Non-conjugated
• Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Species: Human
• Research Area: Immunology
• Target Names: TXK
• Preparation and Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

IF (Immunofluorescence)

(Immunofluorescent analysis of HepG2 cells using AAA233307 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

IHC (Immunohistochemisry)

(Immunohistochemistry of paraffin-embedded human breast cancer using AAA233307 at dilution of 1:100)

IHC (Immunohiostchemistry)

(Immunohistochemistry of paraffin-embedded human lymph node tissue using AAA233307 at dilution of 1:100)

WB (Western Blot)

(Western BlotPositive WB detected in: HEK293 whole cell lysate,Jurkat whole cell lysate,Rat liver tissue,Rat stomach tissueAll lanes: TXK antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 62 kDaObserved band size: 62 kDa)

Related Product Information for anti-TXK antibody

Non-receptor tyrosine kinase that plays a redundant role with ITK in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of TXK to the cell membrane, where it is phosphorylated at Tyr-420. Phosphorylation leads to TXK full activation. Contributes also to signaling from many receptors and participates in multiple downstream pathways, including regulation of the actin cytoskeleton. Like ITK, can phosphorylate PLCG1, leading to its localization in lipid rafts and activation, followed by subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. With PARP1 and EEF1A1, TXK forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFNG to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Phosphorylates both PARP1 and EEF1A1. Phosphorylates also key sites in LCP2 leading to the up-regulation of Th1 preferred cytokine IL-2. Phosphorylates 'Tyr-201' of CTLA4 which leads to the association of PI-3 kinase with the CTLA4 receptor.

NCBI and Uniprot Product Information

• NCBI GI #: 148596974
• NCBI GeneID: 7294
• NCBI Accession #: NP_003319.2
• NCBI GenBank Nucleotide #: NM_003328.2
• UniProt Accession #: P42681; Q14220
• NCBI Official Full Name: tyrosine-protein kinase TXK
• NCBI Official Synonym Full Names: TXK tyrosine kinase
• NCBI Official Symbol: TXK
• NCBI Official Synonym Symbols: RLK; TKL; BTKL; PTK4; PSCTK5
• NCBI Protein Information: tyrosine-protein kinase TXK
• UniProt Protein Name: Tyrosine-protein kinase TXK
• UniProt Gene Name: TXK
• UniProt Synonym Gene Names: PTK4; RLK