IP (Immunoprecipitation)
(Detection of human TXNIP by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TXNIP antibody AAA212142 used for IP at 6 ug/mg lysate. TXNIP was also immunoprecipitated by rabbit anti-TXNIP antibody BL11547, which recognizes an upstream epitope. For blotting immunoprecipitated TXNIP, AAA212142 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human TXNIP Polyclonal Antibody | anti-TXNIP antibody
Rabbit anti-TXNIP Antibody, Affinity Purified
Gene Names
TXNIP; THIF; VDUP1; HHCPA78; EST01027
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
TXNIP, Antibody; Rabbit anti-TXNIP Antibody, Affinity Purified; thioredoxin-interacting protein; vitamin D3 up-regulated protein 1; upregulated by 1,25-dihydroxyvitamin D-3; Thioredoxin-binding protein 2; THIF; ARRDC6; HHCPA78; VDUP1; EST01027; thioredoxin binding protein 2; Thioredoxin-interacting protein; thioredoxin interacting protein; anti-TXNIP antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-TXNIP antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Predicted Reactivity
Rat
Immunogen
Between 341 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human TXNIP by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TXNIP antibody AAA212142 used for IP at 6 ug/mg lysate. TXNIP was also immunoprecipitated by rabbit anti-TXNIP antibody BL11547, which recognizes an upstream epitope. For blotting immunoprecipitated TXNIP, AAA212142 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human TXNIP by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TXNIP antibody AAA212142 used for IP at 6 ug/mg lysate. TXNIP was also immunoprecipitated by rabbit anti-TXNIP antibody BL11547, which recognizes an upstream epitope. For blotting immunoprecipitated TXNIP, AAA212142 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of human TXNIP by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-TXNIP (Cat. No. AAA212142 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human TXNIP by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-TXNIP (Cat. No. AAA212142 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-TXNIP antibody
Thioredoxin-interacting protein (TXNIP) may act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. TXNIP interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Also, TXNIP functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9H3M7].
Product Categories/Family for anti-TXNIP antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,661 Da
NCBI Official Full Name
thioredoxin-interacting protein
NCBI Official Synonym Full Names
thioredoxin interacting protein
NCBI Official Symbol
TXNIP
NCBI Official Synonym Symbols
THIF; VDUP1; HHCPA78; EST01027
NCBI Protein Information
thioredoxin-interacting protein; thioredoxin binding protein 2; thioredoxin-binding protein 2; vitamin D3 up-regulated protein 1; upregulated by 1,25-dihydroxyvitamin D-3
UniProt Protein Name
Thioredoxin-interacting protein
UniProt Gene Name
TXNIP
UniProt Synonym Gene Names
VDUP1
UniProt Entry Name
TXNIP_HUMAN
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Product Notes
The TXNIP txnip (Catalog #AAA212142) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-TXNIP Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TXNIP can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the TXNIP txnip for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TXNIP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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