Rabbit anti-Human, Mouse TYK2 Polyclonal Antibody | anti-TYK2 antibody

TYK2 Antibody (C-term)

Cat. Num. AAA28807

• Gene Names: TYK2; JTK1; IMD35
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: TYK2, Antibody; TYK2 Antibody (C-term); Non-receptor tyrosine-protein kinase TYK2; TYK2; anti-TYK2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This TYK2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 887-922 amino acids from the C-terminal region of human TYK2.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Length: 1187

• Applicable Applications for anti-TYK2 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
• Application Notes: WB: 1:1000; IHC-P: 1:25
• Immunogen Region: 887-922
• Tissue Location: Observed in all cell lines analyzed. Expressed in a variety of lymphoid and non-lymphoid cell lines
• Function: Tyrosine kinase of the non-receptor type involved in numerous cytokines and interferons signaling, which regulates cell growth, development, cell migration, innate and adaptive immunity (PubMed:10542297, PubMed:10995743, PubMed:7657660, PubMed:7813427, PubMed:8232552). Plays both structural and catalytic roles in numerous interleukins and interferons (IFN-alpha/beta) signaling (PubMed:10542297). Associates with heterodimeric cytokine receptor complexes and activates STAT family members including STAT1, STAT3, STAT4 or STAT6 (PubMed:10542297, PubMed:7638186). The heterodimeric cytokine receptor complexes are composed of (1) a TYK2-associated receptor chain (IFNAR1, IL12RB1, IL10RB or IL13RA1), and (2) a second receptor chain associated either with JAK1 or JAK2 (PubMed:10542297, PubMed:25762719, PubMed:7526154, PubMed:7813427). In response to cytokine-binding to receptors, phosphorylates and activates receptors (IFNAR1, IL12RB1, IL10RB or IL13RA1), creating docking sites for STAT members (PubMed:7526154, PubMed:7657660). In turn, recruited STATs are phosphorylated by TYK2 (or JAK1/JAK2 on the second receptor chain), form homo- and heterodimers, translocate to the nucleus, and regulate cytokine/growth factor responsive genes (PubMed:10542297, PubMed:25762719, PubMed:7657660). Negatively regulates STAT3 activity by promototing phosphorylation at a specific tyrosine that differs from the site used for signaling (PubMed:29162862).
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistochemistry-Paraffin)

(Immunohistochemical analysis of paraffin-embedded H. liver section using TYK2 Antibody (C-term) . AAA28807 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)

WB (Western Blot)

(Western blot analysis of lysates from Hela cell line,mouse liver tissue lysate(from left to right), using TYK2 Antibody (C-term). AAA28807 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.)

Related Product Information for anti-TYK2 antibody

Probably involved in intracellular signal transduction by being involved in the initiation of type I IFN signaling. Phosphorylates the interferon-alpha/beta receptor alpha chain.

References

Firmbach-Kraft I.,et al.Oncogene 5:1329-1336(1990). Velazquez L.,et al.Cell 70:313-322(1992). Krolewski J.J.,et al.Oncogene 5:277-282(1990). Partanen J.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:8913-8917(1990). Colamonici O.,et al.Mol. Cell. Biol. 14:8133-8142(1994).

NCBI and Uniprot Product Information

• NCBI GI #: 187608615
• NCBI GeneID: 7297
• NCBI Accession #: NP_003322.3
• NCBI GenBank Nucleotide #: NP_003322.3
• UniProt Accession #: P29597; Q9R117
• Molecular Weight: H=134; M=133kDa
• NCBI Official Full Name: non-receptor tyrosine-protein kinase TYK2
• NCBI Official Synonym Full Names: tyrosine kinase 2
• NCBI Official Symbol: TYK2
• NCBI Official Synonym Symbols: JTK1; IMD35
• NCBI Protein Information: non-receptor tyrosine-protein kinase TYK2
• UniProt Protein Name: Non-receptor tyrosine-protein kinase TYK2
• UniProt Gene Name: TYK2
• UniProt Entry Name: TYK2_HUMAN