Rabbit anti-Human Tyrosine Hydroxylase Polyclonal Antibody | anti-TH antibody

Anti-Tyrosine Hydroxylase Antibody, Rabbit Polyclonal

Cat. Num. AAA254308

• Gene Names: TH; TYH; DYT14; DYT5b
• Reactivity: Human
• Applications: Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: Tyrosine Hydroxylase, Antibody; Anti-Tyrosine Hydroxylase Antibody, Rabbit Polyclonal; Tyrosine Hydroxylase/TH Antibody, Rabbit PAb, Antigen Affinity Purified; tyrosine hydroxylase; Anti-DYT14 Antibody; Anti-DYT5b Antibody; Anti-TYH Antibody; anti-TH antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Tyrosine Hydroxylase/TH
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-TH antibody: IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human Tyrosine Hydroxylase/TH
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Tyrosine Hydroxylase/TH, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(TH was immunoprecipitated using:Lane A:0.5 mg PC-3 Whole Cell Lysate0.5 uL anti-TH rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-TH rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 59 kDaObserved band size: 99 kDa)

WB (Western Blot)

(Anti-TH rabbit polyclonal antibody at 1:500 dilutionLane A: PC12 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:59 kDaObserved band size:59 kDa)

Related Product Information for anti-TH antibody

Tyrosine hydroxylase (TH) is a rate-limiting enzyme in catecholamine synthesis. Tyrosine hydroxylase activity is modulated by protein-protein interactions with enzymes in the same pathway or the tetrahydrobiopterin pathway, structural proteins considered to be chaperones that mediate the neuron's oxidative state, and the protein that transfers dopamine into secretory vesicles. It is phosphorylated at serine (Ser) residues Ser8, Ser19, Ser31 and Ser40 in vitro. The phosphorylation of tyrosine hydroxylase at Ser19 or Ser8 has no direct effect on tyrosine hydroxylase activity. As tyrosine hydroxylase (TH) catalyses the formation of L-DOPA, the rate-limiting step in the biosynthesis of DA, the Parkinson's disease (PD) can be considered as a TH-deficiency syndrome of the striatum. A direct pathogenetic role of TH has also been suggested, as the enzyme is a source of reactive oxygen species (ROS) in vitro and a target for radical-mediated oxidative injury. Recently, it has been demonstrated that L-DOPA is effectively oxidized by mammalian Tyrosine hydroxylase in vitro, possibly contributing to the cytotoxic effects of DOPA.

Product Categories/Family for anti-TH antibody

Neurotransmitter Associated Enzymes; G Protein-coupled Receptors Signaling

References

Daubner SC, et al. (2011) Tyrosine hydroxylase and regulation of dopamine synthesis. Arch Biochem Biophys. 508(1): 1-12.Dunkley PR, et al. (2004) Tyrosine hydroxylase phosphorylation: regulation and consequences. J Neurochem. 91(5): 1025-43.Haavik J, et al. (1998) Tyrosine hydroxylase and Parkinson's disease. Mol Neurobiol. 16(3): 285-309.

NCBI and Uniprot Product Information

• NCBI GI #: 88900503
• NCBI GeneID: 7054
• NCBI Accession #: NP_000351.2
• NCBI GenBank Nucleotide #: NM_000360.3
• UniProt Accession #: P07101; Q15585; Q15588; Q15589; B7ZL70
• Molecular Weight: 58,600 Da
• NCBI Official Full Name: tyrosine 3-monooxygenase isoform b
• NCBI Official Synonym Full Names: tyrosine hydroxylase
• NCBI Official Symbol: TH
• NCBI Official Synonym Symbols: TYH; DYT14; DYT5b
• NCBI Protein Information: tyrosine 3-monooxygenase; dystonia 14; tyrosine 3-hydroxylase
• UniProt Protein Name: Tyrosine 3-monooxygenase
• UniProt Gene Name: TH
• UniProt Synonym Gene Names: TYH; TH
• UniProt Entry Name: TY3H_HUMAN