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WB (Western Blot)
(Western blot analysis of U2AF1 (arrow) using rabbit polyclonal U2AF1 Antibody (Center Y114). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the U2AF1 gene.)
Rabbit U2AF1 Polyclonal Antibody | anti-U2AF1 antibody
U2AF1 Antibody (Center Y114)
Gene Names
U2AF1; RN; FP793; U2AF35; U2AFBP; RNU2AF1
Reactivity
Human (Predicted Reactivity: Bovine, Mouse)
Applications
Western Blot, Immunohistochemistry, ELISA, Flow Cytometry, Functional Assay
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
U2AF1, Antibody; U2AF1 Antibody (Center Y114); Splicing factor U2AF 35 kDa subunit; U2 auxiliary factor 35 kDa subunit; U2 small nuclear RNA auxiliary factor 1; U2 snRNP auxiliary factor small subunit; U2AF1; U2AF35; U2AFBP; anti-U2AF1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This U2AF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99-125 amino acids from the Central region of human U2AF1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
99-125
Sequence Length
240
Applicable Applications for anti-U2AF1 antibody
WB (Western Blot), IHC (Immunohistochemistry), ELISA, FCM/FACS (Flow Cytometry)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of U2AF1 (arrow) using rabbit polyclonal U2AF1 Antibody (Center Y114). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the U2AF1 gene.)
WB (Western Blot)
(Western blot analysis of U2AF1 (arrow) using rabbit polyclonal U2AF1 Antibody (Center Y114). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the U2AF1 gene.)
FCM/FACS (Flow Cytometry)
(U2AF1 Antibody (Center Y114) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(U2AF1 Antibody (Center Y114) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with U2AF1 Antibody (Center Y114) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with U2AF1 Antibody (Center Y114) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of anti-U2AF1 Antibody (Center Y114) in Ramos cell line lysates (35ug/lane).U2AF1(arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of anti-U2AF1 Antibody (Center Y114) in Ramos cell line lysates (35ug/lane).U2AF1(arrow) was detected using the purified Pab.)
Related Product Information for anti-U2AF1 antibody
U2AF1 belongs to the splicing factor SR family. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers.
References
Rino,J., Mol. Cell. Biol. 28 (9), 3045-3057 (2008) Little,J.T., J. Biol. Chem. 283 (13), 8145-8152 (2008) Pacheco,T.R., Mol. Cell. Biol. 26 (21), 8183-8190 (2006)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27872
NCBI Official Full Name
splicing factor U2AF 35 kDa subunit isoform b
NCBI Official Synonym Full Names
U2 small nuclear RNA auxiliary factor 1
NCBI Official Symbol
U2AF1
NCBI Official Synonym Symbols
RN; FP793; U2AF35; U2AFBP; RNU2AF1
NCBI Protein Information
splicing factor U2AF 35 kDa subunit
UniProt Protein Name
Splicing factor U2AF 35 kDa subunit
UniProt Gene Name
U2AF1
UniProt Synonym Gene Names
U2AF35; U2AFBP
UniProt Entry Name
U2AF1_HUMAN
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Product Notes
The U2AF1 u2af1 (Catalog #AAA284629) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 99-125. The U2AF1 Antibody (Center Y114) reacts with Human (Predicted Reactivity: Bovine, Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's U2AF1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the U2AF1 u2af1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "U2AF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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