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product-image-AAA212503_WB13.jpg WB (Western Blot) (Detection of human UBA5 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, U2OS, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-UBA5 antibody AAA212503 (lot AAA212503-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

Rabbit anti-Human UBA5 Polyclonal Antibody | anti-UBA5 antibody

Rabbit anti-UBA5 Antibody, Affinity Purified

Gene Names
UBA5; THIFP1; UBE1DC1
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
UBA5, Antibody; Rabbit anti-UBA5 Antibody, Affinity Purified; ubiquitin-activating enzyme 5; UFM1-activating enzyme; ubiquitin-like modifier-activating enzyme 5; ubiquitin-activating enzyme E1 domain-containing protein 1; UBE1DC1; UBA5, ubiquitin-activating enzyme E1 homolog; THIFP1; SCAR24; EIEE44; ubiquitin-activating enzyme E1-domain containing 1; Ubiquitin-like modifier-activating enzyme 5; ubiquitin like modifier activating enzyme 5; anti-UBA5 antibody
Ordering
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-UBA5 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 300 and 350
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human UBA5 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, U2OS, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-UBA5 antibody AAA212503 (lot AAA212503-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

product-image-AAA212503_WB13.jpg WB (Western Blot) (Detection of human UBA5 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, U2OS, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-UBA5 antibody AAA212503 (lot AAA212503-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

IP (Immunoprecipitation)

(Detection of human UBA5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-UBA5 antibody AAA212503 (lot AAA212503-2) used for IP at 6 ug per reaction. UBA5 was also immunoprecipitated by a previous lot of this antibody (lot AAA212503-1). For blotting immunoprecipitated UBA5, AAA212503 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

product-image-AAA212503_IP15.jpg IP (Immunoprecipitation) (Detection of human UBA5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-UBA5 antibody AAA212503 (lot AAA212503-2) used for IP at 6 ug per reaction. UBA5 was also immunoprecipitated by a previous lot of this antibody (lot AAA212503-1). For blotting immunoprecipitated UBA5, AAA212503 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-UBA5 antibody
Ubiquitin-like modifier activating enzyme 5 (UBA5) is a member of the E1-like ubiquitin-activating enzyme family. This protein activates ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein, via the formation of a high-energy thioester bond [taken from NCBI Entrez Gene (Gene ID: 79876).
Product Categories/Family for anti-UBA5 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
38,537 Da
NCBI Official Full Name
ubiquitin-like modifier-activating enzyme 5 isoform 1
NCBI Official Synonym Full Names
ubiquitin-like modifier activating enzyme 5
NCBI Official Symbol
UBA5
NCBI Official Synonym Symbols
THIFP1; UBE1DC1
NCBI Protein Information
ubiquitin-like modifier-activating enzyme 5; UBA5, ubiquitin-activating enzyme E1 homolog; UFM1-activating enzyme; ubiquitin-activating enzyme 5; ubiquitin-activating enzyme E1 domain-containing protein 1; ubiquitin-activating enzyme E1-domain containing
UniProt Protein Name
Ubiquitin-like modifier-activating enzyme 5
UniProt Gene Name
UBA5
UniProt Synonym Gene Names
UBE1DC1; Ubiquitin-activating enzyme 5
UniProt Entry Name
UBA5_HUMAN

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Product Notes

The UBA5 uba5 (Catalog #AAA212503) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-UBA5 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's UBA5 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the UBA5 uba5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBA5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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