WB (Western Blot)
(Western blot analysis of UBE2D3 (arrow) using rabbit polyclonal UBE2D3 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2D3 gene.)
Rabbit UBE2D3 Polyclonal Antibody | anti-UBE2D3 antibody
UBE2D3 Antibody (C-term)
Gene Names
UBE2D3; UBC4/5; UBCH5C; E2(17)KB3
Reactivity
Human (Predicted Reactivity: Pig, Bovine, Monkey, Mouse, Rat)
Applications
ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
UBE2D3, Antibody; UBE2D3 Antibody (C-term); Ubiquitin-conjugating enzyme E2 D3; Ubiquitin carrier protein D3; Ubiquitin-conjugating enzyme E2(17)KB 3; Ubiquitin-conjugating enzyme E2-17 kDa 3; Ubiquitin-protein ligase D3; UBE2D3; UBC5C; UBCH5C; anti-UBE2D3 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Pig, Bovine, Monkey, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This UBE2D3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-136 amino acids from the C-terminal region of human UBE2D3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
109-136
Sequence Length
147
Applicable Applications for anti-UBE2D3 antibody
ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of UBE2D3 (arrow) using rabbit polyclonal UBE2D3 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2D3 gene.)
WB (Western Blot)
(Western blot analysis of UBE2D3 (arrow) using rabbit polyclonal UBE2D3 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2D3 gene.)
WB (Western Blot)
(UBE2D3 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the UBE2D3 antibody detected the UBE2D3 protein (arrow).)
WB (Western Blot)
(UBE2D3 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the UBE2D3 antibody detected the UBE2D3 protein (arrow).)
Related Product Information for anti-UBE2D3 antibody
The modification of proteins with ubiquitin is an
important cellular mechanism for targeting abnormal or short-lived
proteins for degradation. Ubiquitination involves at least three
classes of enzymes: ubiquitin-activating enzymes, or E1s,
ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein
ligases, or E3s. This gene encodes a member of the E2
ubiquitin-conjugating enzyme family. This enzyme functions in the
ubiquitination of the tumor-suppressor protein p53, which is
induced by an E3 ubiquitin-protein ligase. Multiple spliced
transcript variants have been found for this gene, but the
full-length nature of some variants has not been determined.
important cellular mechanism for targeting abnormal or short-lived
proteins for degradation. Ubiquitination involves at least three
classes of enzymes: ubiquitin-activating enzymes, or E1s,
ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein
ligases, or E3s. This gene encodes a member of the E2
ubiquitin-conjugating enzyme family. This enzyme functions in the
ubiquitination of the tumor-suppressor protein p53, which is
induced by an E3 ubiquitin-protein ligase. Multiple spliced
transcript variants have been found for this gene, but the
full-length nature of some variants has not been determined.
Product Categories/Family for anti-UBE2D3 antibody
References
Kalsi, G., et al. Hum. Mol. Genet. 19(12):2497-2506(2010)
Wu, K., et al. Mol. Cell 37(6):784-796(2010)
Vina-Vilaseca, A., et al. J. Biol. Chem. 285(10):7645-7656(2010)
Markson, G., et al. Genome Res. 19(10):1905-1911(2009)
van Wijk, S.J., et al. Mol. Syst. Biol. 5, 295 (2009) :
Wu, K., et al. Mol. Cell 37(6):784-796(2010)
Vina-Vilaseca, A., et al. J. Biol. Chem. 285(10):7645-7656(2010)
Markson, G., et al. Genome Res. 19(10):1905-1911(2009)
van Wijk, S.J., et al. Mol. Syst. Biol. 5, 295 (2009) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16687
NCBI Official Full Name
ubiquitin-conjugating enzyme E2 D3 isoform 1
NCBI Official Synonym Full Names
ubiquitin-conjugating enzyme E2D 3
NCBI Official Symbol
UBE2D3
NCBI Official Synonym Symbols
UBC4/5; UBCH5C; E2(17)KB3
NCBI Protein Information
ubiquitin-conjugating enzyme E2 D3
UniProt Protein Name
Ubiquitin-conjugating enzyme E2 D3
UniProt Gene Name
UBE2D3
UniProt Synonym Gene Names
UBC5C; UBCH5C
UniProt Entry Name
UB2D3_HUMAN
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Product Notes
The UBE2D3 ube2d3 (Catalog #AAA284526) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 109-136. The UBE2D3 Antibody (C-term) reacts with Human (Predicted Reactivity: Pig, Bovine, Monkey, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's UBE2D3 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the UBE2D3 ube2d3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBE2D3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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