WB (Western Blot)
(Western blot analysis of extracts of mouse embryo tissue, using UBE2E2 antibody.)
Rabbit UBE2E2 Polyclonal Antibody | anti-UBE2E2 antibody
UBE2E2 Antibody
Gene Names
UBE2E2; UBCH8
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
UBE2E2, Antibody; UBE2E2 Antibody; FLJ25157; UB2E2_HUMAN; UBC4/5 homolog yeast; UBCH 8; UbcH8; Ube2e2; Ubiquitin carrier protein E2; Ubiquitin conjugating enzyme E2 E2; Ubiquitin conjugating enzyme E2E 2 (homologous to yeast UBC4/5); Ubiquitin conjugating enzyme E2E 2 (UBC4/5 homolog yeast); Ubiquitin conjugating enzyme E2E 2; Ubiquitin protein ligase E2; Ubiquitin-conjugating enzyme E2 E2; Ubiquitin-protein ligase E2; anti-UBE2E2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
UBE2E2 antibody detects endogenous levels of UBE2E2.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
201
Applicable Applications for anti-UBE2E2 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human UBE2E2.
Predicted Cross Reactivity
Zebrafish, Chicken, Xenopus
Similarity
Zebrafish (92%), Chicken (100%), Xenopus (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts of mouse embryo tissue, using UBE2E2 antibody.)
WB (Western Blot)
(Western blot analysis of extracts of mouse embryo tissue, using UBE2E2 antibody.)
IHC (Immunohistochemistry)
(AAA323316 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA323316 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-UBE2E2 antibody
Function: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. Catalyzes the ISGylation of influenza A virus NS1 protein.
Post-translational Modifications: Autoubiquitinated in vitro.
Similarity: Belongs to the ubiquitin-conjugating enzyme family.
Post-translational Modifications: Autoubiquitinated in vitro.
Similarity: Belongs to the ubiquitin-conjugating enzyme family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 22 kDa
Predicted: 23 kDa
Predicted: 23 kDa
NCBI Official Full Name
ubiquitin-conjugating enzyme E2 E2
NCBI Official Synonym Full Names
ubiquitin conjugating enzyme E2 E2
NCBI Official Symbol
UBE2E2
NCBI Official Synonym Symbols
UBCH8
NCBI Protein Information
ubiquitin-conjugating enzyme E2 E2
UniProt Protein Name
Ubiquitin-conjugating enzyme E2 E2
UniProt Gene Name
UBE2E2
UniProt Synonym Gene Names
UBCH8
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Product Notes
The UBE2E2 ube2e2 (Catalog #AAA323316) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UBE2E2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's UBE2E2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the UBE2E2 ube2e2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBE2E2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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