WB (Western Blot)
(Anti-UBE2H rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:21 kDaObserved band size:21 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Human Ube2H Polyclonal Antibody | anti-UBE2H antibody
Anti-Ube2H Antibody, Rabbit Polyclonal
Gene Names
UBE2H; GID3; UBC8; UBCH; UBCH2; E2-20K
Reactivity
Human
Applications
Immunoprecipitation, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
Ube2H, Antibody; Anti-Ube2H Antibody, Rabbit Polyclonal; UBE2H Antibody, Rabbit PAb, Antigen Affinity Purified; ubiquitin-conjugating enzyme E2H; Anti-E2-20K Antibody; Anti-GID3 Antibody; Anti-UBC8 Antibody; Anti-UBCH Antibody; Anti-UBCH2 Antibody; anti-UBE2H antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human UBE2H
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-UBE2H antibody
IP (Immunoprecipitation), ELISA, WB (Western Blot)
Immunogen
Recombinant Human UBE2H protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human UBE2H (rh UBE2H). UBE2H specific IgG was purified by Human UBE2H affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Anti-UBE2H rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:21 kDaObserved band size:21 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-UBE2H rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:21 kDaObserved band size:21 kDa(We are unsure as to the identity of these extra bands.))
IP (Immunoprecipitation)
(UBE2H was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate2 uL anti-UBE2H rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-UBE2H rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 22 kDaObserved band size: 22 kDa)
IP (Immunoprecipitation)
(UBE2H was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate2 uL anti-UBE2H rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-UBE2H rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 22 kDaObserved band size: 22 kDa)
Related Product Information for anti-UBE2H antibody
UBE2H is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation, ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction, and thereby the fate of the substrate, is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space, a highly sophisticated conjugation machinery has been developed. In a sequential manner, ubiquitin becomes activated by a ubiquitin-activating enzyme (E1), which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next, ubiquitin-loaded E2s are interacting with ubiquitin-protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system, wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans.
References
Sjoerd J L van Wijk, et al. (2009) A comprehensive framework of E2-RING E3 interactions of the human ubiquitin-proteasome system. Mol Syst Biol. 5: 317.Nandi D, et al. (2006) The ubiquitin-proteasome system. Journal of biosciences. 31 (1): 137-55.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
183
NCBI Official Full Name
Ubiquitin-conjugating enzyme E2 H
NCBI Official Synonym Full Names
ubiquitin-conjugating enzyme E2H
NCBI Official Symbol
UBE2H
NCBI Official Synonym Symbols
GID3; UBC8; UBCH; UBCH2; E2-20K
NCBI Protein Information
ubiquitin-conjugating enzyme E2 H; ubiquitin-protein ligase H; ubiquitin carrier protein H; GID complex subunit 3, UBC8 homolog; ubiquitin-conjugating enzyme E2-20K; ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast); ubiquitin-conjugating enzyme E2H
UniProt Protein Name
Ubiquitin-conjugating enzyme E2 H
UniProt Gene Name
UBE2H
UniProt Entry Name
UBE2H_HUMAN
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Product Notes
The UBE2H ube2h (Catalog #AAA255615) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Ube2H Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Ube2H can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the UBE2H ube2h for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ube2H, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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