WB (Western Blot)
(UBE3C Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the UBE3C antibody detected the UBE3C protein (arrow).)
Rabbit anti-Human UBE3C Polyclonal Antibody | anti-UBE3C antibody
UBE3C Antibody (Center)
Gene Names
UBE3C; HECTH2
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
UBE3C, Antibody; UBE3C Antibody (Center); Ubiquitin-protein ligase E3C; 632-; HectH2; UBE3C; KIAA0010; KIAA10; anti-UBE3C antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This UBE3C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 549-578 amino acids from the Central region of human UBE3C.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.27 (varies by lot)
Sequence Positions
549-578
Sequence Length
1083
Applicable Applications for anti-UBE3C antibody
ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Cellular Location
Nucleus
Tissue Location
Highly expressed in skeletal muscle. Detected at much lower levels in kidney and pancreas.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(UBE3C Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the UBE3C antibody detected the UBE3C protein (arrow).)
WB (Western Blot)
(UBE3C Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the UBE3C antibody detected the UBE3C protein (arrow).)
WB (Western Blot)
(Western blot analysis of lysates from Hela cell line, mouse brain tissue lysate, HepG2 cell line (from left to right), using UBE3CAntibody(Center). AAA288782 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from Hela cell line, mouse brain tissue lysate, HepG2 cell line (from left to right), using UBE3CAntibody(Center). AAA288782 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Related Product Information for anti-UBE3C antibody
E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'-or 'Lys-48'-linked polyubiquitin chains. Has preference for 'Lys-48' linkages. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo.
Product Categories/Family for anti-UBE3C antibody
References
Nomura N., et al. DNA Res. 1:27-35(1994).
Hillier L.W., et al. Nature 424:157-164(2003).
Scherer S.W., et al. Science 300:767-772(2003).
Mural R.J., et al. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
You J., et al. J. Biol. Chem. 276:19871-19878(2001).
Hillier L.W., et al. Nature 424:157-164(2003).
Scherer S.W., et al. Science 300:767-772(2003).
Mural R.J., et al. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
You J., et al. J. Biol. Chem. 276:19871-19878(2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
123923
NCBI Official Full Name
ubiquitin-protein ligase E3C
NCBI Official Synonym Full Names
ubiquitin protein ligase E3C
NCBI Official Symbol
UBE3C
NCBI Official Synonym Symbols
HECTH2
NCBI Protein Information
ubiquitin-protein ligase E3C
UniProt Protein Name
Ubiquitin-protein ligase E3C
UniProt Gene Name
UBE3C
UniProt Synonym Gene Names
KIAA0010; KIAA10
UniProt Entry Name
UBE3C_HUMAN
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Product Notes
The UBE3C ube3c (Catalog #AAA288782) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 549-578. The UBE3C Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's UBE3C can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the UBE3C ube3c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBE3C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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