WB (Western Blot)
(Detection of human UBE4A by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-UBE4A antibody AAA212601 (lot AAA212601-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human UBE4A Polyclonal Antibody | anti-UBE4A antibody
Rabbit anti-UBE4A Antibody, Affinity Purified
Gene Names
UBE4A; E4; UFD2; UBOX2; KIAA0126; MGC133315
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
UBE4A, Antibody; Rabbit anti-UBE4A Antibody, Affinity Purified; UFD2; UBOX2; ubiquitination factor E4A (UFD2 homolog, yeast); ubiquitin conjugation factor E4 A; E4; RING-type E3 ubiquitin transferase E4 A; Ubiquitin conjugation factor E4 A; ubiquitination factor E4A; anti-UBE4A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-UBE4A antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, Orangutan
Immunogen
Between 1016 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human UBE4A by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-UBE4A antibody AAA212601 (lot AAA212601-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human UBE4A by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-UBE4A antibody AAA212601 (lot AAA212601-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human UBE4A by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-UBE4A antibody AAA212601 (lot AAA212601-1) used for IP at 6 ug per reaction. UBE4A was also immunoprecipitated by rabbit anti-UBE4A antibody For blotting immunoprecipitated UBE4A, AAA212601 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human UBE4A by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-UBE4A antibody AAA212601 (lot AAA212601-1) used for IP at 6 ug per reaction. UBE4A was also immunoprecipitated by rabbit anti-UBE4A antibody For blotting immunoprecipitated UBE4A, AAA212601 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-UBE4A antibody
Ubiquitin conjugation factor E4 A (UBE4A) binds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3 (By similarity) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q14139].
Product Categories/Family for anti-UBE4A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
122,561 Da
NCBI Official Full Name
ubiquitin conjugation factor E4 A isoform 2
NCBI Official Synonym Full Names
ubiquitination factor E4A
NCBI Official Symbol
UBE4A
NCBI Official Synonym Symbols
E4; UFD2; UBOX2; KIAA0126; MGC133315
NCBI Protein Information
ubiquitin conjugation factor E4 A; OTTHUMP00000237916; OTTHUMP00000237917; ubiquitination factor E4A (UFD2 homolog, yeast)
UniProt Protein Name
Ubiquitin conjugation factor E4 A
UniProt Gene Name
UBE4A
UniProt Entry Name
UBE4A_HUMAN
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Product Notes
The UBE4A ube4a (Catalog #AAA212601) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-UBE4A Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's UBE4A can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the UBE4A ube4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBE4A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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