Rabbit anti-Human UBLCP1 Polyclonal Antibody | anti-UBLCP1 antibody

Anti-UBLCP1 Antibody, Rabbit Polyclonal

Cat. Num. AAA256469

• Reactivity: Human
• Applications: Immunoprecipitation, Immunohistochemistry, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: UBLCP1, Antibody; Anti-UBLCP1 Antibody, Rabbit Polyclonal; UBLCP1 Antibody, Rabbit PAb, Antigen Affinity Purified; ubiquitin like domain containing CTD phosphatase 1; Anti-CPUB1 Antibody; anti-UBLCP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human UBLCP1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-UBLCP1 antibody: IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: E Coli-derived Human UBLCP1 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human UBLCP1 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(UBLCP1 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell Lysate4 uL anti-UBLCP1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-UBLCP1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 43 kDaObserved band size :43 kDa)

IHC (Immunohiostchemistry)

(Immunochemical staining of human UBLCP1 in human testis with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot)

(Anti-UBLCP1 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLane B: HeLa Whole Cell LysateLane C: K562 Whole Cell LysateLane D: U251MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:37 kDaObserved band size:43 kDa)

Related Product Information for anti-UBLCP1 antibody

Ubiquitin-like domain-containing C-terminal domain phosphatase 1 (UBLCP1), an FCP/SCP phosphatase family member, was identified as the first proteasome phosphatase. UBLCP1 binds to proteasome subunit Rpn1 and dephosphorylates the proteasome in vitro. UBLCP1 is a 26S proteasome phosphatase that regulates nuclear proteasome activity. UBLCP1 has been implicated as a negative regulator of the proteasome, a key mediator in the ubiquitin-dependent protein degradation. UBLCP1 directly interacts with the proteasome via its UBL domain and is exclusively localized in the nucleus. UBLCP1 dephosphorylates the 26S proteasome and inhibits the proteasome activity in vitro. Knockdown of UBLCP1 in cells promotes 26S proteasome assembly and selectively enhances nuclear proteasome activity. Small molecule inhibitors that block UBLCP1 activity would be valuable as research tools and potential therapeutics for human diseases caused by the cellular accumulation of misfold/damaged proteins.

References

Sun S, et al. (2017) Phosphatase ublcp1 controls proteasome assembly. Open Biol 7 (5): 170042.He Y, et al. (2015) A potent and selective inhibitor for the ublcp1 proteasome phosphatase. Bioorg Med Chem 23 (12): 2798-2809.Guo X, et al. (2011) Ublcp1 is a 26s proteasome phosphatase that regulates nuclear proteasome activity. Proc Natl Acad Sci U S A 108 (46): 18649-18654.