IHC (Immunohistochemisry)
(UBP1 Antibody (Center) (AAA284114)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UBP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit UBP1 Polyclonal Antibody | anti-UBP1 antibody
UBP1 Antibody (Center)
Gene Names
UBP1; LBP1A; LBP1B; LBP-1B; LBP-1a
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Western Blot, Immunofluorescence, Immunohistochemistry, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
UBP1, Antibody; UBP1 Antibody (Center); Upstream-binding protein 1; Transcription factor LBP-1; UBP1; LBP1; anti-UBP1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This UBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 266-295 amino acids from the Central region of human UBP1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
266-295
Sequence Length
504
Applicable Applications for anti-UBP1 antibody
WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(UBP1 Antibody (Center) (AAA284114)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UBP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(UBP1 Antibody (Center) (AAA284114)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UBP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(UBP1 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the UBP1 antibody detected the UBP1 protein (arrow).)
WB (Western Blot)
(UBP1 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the UBP1 antibody detected the UBP1 protein (arrow).)
IF (Immunofluorescence)
(Fluorescent image of A549 cell stained with UBP1 Antibody (Center). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with UBP1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).UBP1 immunoreactivity is localized to Nucleus significantly.)
IF (Immunofluorescence)
(Fluorescent image of A549 cell stained with UBP1 Antibody (Center). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with UBP1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).UBP1 immunoreactivity is localized to Nucleus significantly.)
Related Product Information for anti-UBP1 antibody
UBP1 functions as a transcriptional activator in a promoter context-dependent manner. Modulates the placental expression of CYP11A1. Involved in regulation of the alpha-globin gene in erythroid cells. Activation of the alpha-globin promoter in erythroid cells is via synergistic interaction with TFCP2 (By similarity). Involved in regulation of the alpha-globin gene in erythroid cells. Binds strongly to sequences around the HIV-1 initiation site and weakly over the TATA-box. Represses HIV-1 transcription by inhibiting the binding of TFIID to the TATA-box.
References
Katsura, A., et al. Genes Cells 14(10):1183-1196(2009)
Koutnikova, H., et al. PLoS Genet. 5 (8), E1000591 (2009) :
Henderson, Y.C., et al. DNA Cell Biol. 27(2):71-79(2008)
Huang, N., et al. Mol. Endocrinol. 19(2):409-420(2005)
Huang, N., et al. J. Biol. Chem. 275(4):2852-2858(2000)
Koutnikova, H., et al. PLoS Genet. 5 (8), E1000591 (2009) :
Henderson, Y.C., et al. DNA Cell Biol. 27(2):71-79(2008)
Huang, N., et al. Mol. Endocrinol. 19(2):409-420(2005)
Huang, N., et al. J. Biol. Chem. 275(4):2852-2858(2000)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
60491
NCBI Official Full Name
upstream-binding protein 1 isoform LBP-1a
NCBI Official Synonym Full Names
upstream binding protein 1 (LBP-1a)
NCBI Official Symbol
UBP1
NCBI Official Synonym Symbols
LBP1A; LBP1B; LBP-1B; LBP-1a
NCBI Protein Information
upstream-binding protein 1
UniProt Protein Name
Upstream-binding protein 1
UniProt Gene Name
UBP1
UniProt Synonym Gene Names
LBP1
UniProt Entry Name
UBIP1_HUMAN
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Product Notes
The UBP1 ubp1 (Catalog #AAA284114) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 266-295. The UBP1 Antibody (Center) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's UBP1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the UBP1 ubp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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