IHC (Immunohistochemisry)
(UGT8 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UGT8 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit UGT8 Polyclonal Antibody | anti-UGT8 antibody
UGT8 antibody (Center)
Gene Names
UGT8; CGT; UGT4
Reactivity
Human, mouse (Predicted Reactivity: Rat)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
UGT8, Antibody; UGT8 antibody (Center); 2-hydroxyacylsphingosine 1-beta-galactosyltransferase; Ceramide UDP-galactosyltransferase; Cerebroside synthase; UDP-galactose-ceramide galactosyltransferase; UGT8; CGT; UGT4; anti-UGT8 antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This UGT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-393 amino acids from the Central region of human UGT8.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
366-393
Sequence Length
541
Applicable Applications for anti-UGT8 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(UGT8 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UGT8 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(UGT8 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UGT8 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(UGT8 antibody (Center) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the UGT8 antibody detected the UGT8 protein (arrow).)
WB (Western Blot)
(UGT8 antibody (Center) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the UGT8 antibody detected the UGT8 protein (arrow).)
WB (Western Blot)
(Anti-UGT8 antibody (Center)at 1:2000 dilution + human heart lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 61 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-UGT8 antibody (Center)at 1:2000 dilution + human heart lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 61 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-UGT8 antibody
Galactocerebrosides are abundant sphingolipids of the
myelin membrane of the central nervous system and peripheral
nervous system and are also present in small amounts in kidney. The
key enzymatic step in the biosynthesis of galactocerebrosides
consists of the transfer of galactose to ceramide catalyzed by
UDP-galactose ceramide galactosyltransferase (CGT, EC 2.4.1.45).
The enzyme encoded by the CGT gene is the first involved in complex
lipid biosynthesis in the myelinating oligodendrocyte.[supplied by
OMIM].
myelin membrane of the central nervous system and peripheral
nervous system and are also present in small amounts in kidney. The
key enzymatic step in the biosynthesis of galactocerebrosides
consists of the transfer of galactose to ceramide catalyzed by
UDP-galactose ceramide galactosyltransferase (CGT, EC 2.4.1.45).
The enzyme encoded by the CGT gene is the first involved in complex
lipid biosynthesis in the myelinating oligodendrocyte.[supplied by
OMIM].
Product Categories/Family for anti-UGT8 antibody
References
Dziecedil Giel, P., et al. Br. J. Cancer 103(4):524-531(2010)
Kalsi, G., et al. Hum. Mol. Genet. 19(12):2497-2506(2010)
Ross, C.J., et al. Nat. Genet. 41(12):1345-1349(2009)
Ruckhaberle, E., et al. J. Cancer Res. Clin. Oncol. 135(8):1005-1013(2009)
Saito, A., et al. J. Hum. Genet. 54(6):317-323(2009)
Kalsi, G., et al. Hum. Mol. Genet. 19(12):2497-2506(2010)
Ross, C.J., et al. Nat. Genet. 41(12):1345-1349(2009)
Ruckhaberle, E., et al. J. Cancer Res. Clin. Oncol. 135(8):1005-1013(2009)
Saito, A., et al. J. Hum. Genet. 54(6):317-323(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61438
NCBI Official Full Name
2-hydroxyacylsphingosine 1-beta-galactosyltransferase
NCBI Official Synonym Full Names
UDP glycosyltransferase 8
NCBI Official Symbol
UGT8
NCBI Official Synonym Symbols
CGT; UGT4
NCBI Protein Information
2-hydroxyacylsphingosine 1-beta-galactosyltransferase
UniProt Protein Name
2-hydroxyacylsphingosine 1-beta-galactosyltransferase
UniProt Gene Name
UGT8
UniProt Synonym Gene Names
CGT; UGT4
UniProt Entry Name
CGT_HUMAN
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Product Notes
The UGT8 ugt8 (Catalog #AAA283927) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 366-393. The UGT8 antibody (Center) reacts with Human, mouse (Predicted Reactivity: Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's UGT8 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the UGT8 ugt8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UGT8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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