WB (Western Blot)
(Western blot analysis of Rat Brain cell lysates showing detection of ~112.6kDa ULK1 protein using Rabbit Anti-ULK1 Polyclonal Antibody (SPC-640). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 2% GE Healthcare Blocker (RT, 60 minutes). Primary Antibody: Rabbit Anti-ULK1 Polyclonal Antibody (SPC-640) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1/2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~112.6kDa.)
Rabbit ULK1 Polyclonal Antibody | anti-ULK1 antibody
ULK1 Antibody: ATTO 390
Gene Names
ULK1; ATG1; ATG1A; UNC51; hATG1; Unc51.1
Reactivity
Human; Mouse; Rat
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Peptide Affinity Purified
Synonyms
ULK1, Antibody; ULK1 Antibody: ATTO 390; Rabbit Anti-Human ULK1 Polyclonal; Unc-51 like kinase 1 (C. elegans) Antibody; Autophagy related protein 1 homolog Antibody; Autophagy-related protein 1 homolog Antibody; FLJ38455 Antibody; Serine/threonine-protein kinase ULK1 Antibody; Unc51.1 Antibody; ATG1A Antibody; UNC51; C. elegans; homolog of Antibody; EC:2.7.11.1 Antibody; Serine/threonine protein kinase Unc51.1 Antibody; FLJ46475 Antibody; ATG 1 Antibody; hATG1 Antibody; ATG1 Antibody; Unc-51-like kinase 1 Antibody; Serine/threonine protein kinase ULK1 Antibody; ULK1 Antibody; Unc 51 like kinase 1 Antibody; KIAA0722 Antibody; UNC 51 Antibody; ULK 1 Antibody; ULK1_HUMAN Antibody; UNC51 Antibody; Unc 51 (C. elegans) like kinase 1 Antibody; ATG1 autophagy related 1 homolog Antibody; anti-ULK1 antibody
Host
Rabbit
Reactivity
Human; Mouse; Rat
Clonality
Polyclonal
Specificity
Detects ~100 kDa.
Purity/Purification
Peptide Affinity Purified
Concentration
1 mg/ml (varies by lot)
Sequence Length
1050
Applicable Applications for anti-ULK1 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Target
ULK1
Immunogen
Synthetic peptide from the mid-protein of Human ULK1 (aa. 567-577)
Immunogen Species
Human
Cellular Localization
Cytoplasm; Cytosol; Preautophagosomal Structure
Conjugate
ATTO 390
Storage Buffer
PBS, 50% glycerol, 0.09% sodium azide
Tissue Specificity
Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.
Preparation and Storage
Store at -20 degree C.
WB (Western Blot)
(Western blot analysis of Rat Brain cell lysates showing detection of ~112.6kDa ULK1 protein using Rabbit Anti-ULK1 Polyclonal Antibody (SPC-640). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 2% GE Healthcare Blocker (RT, 60 minutes). Primary Antibody: Rabbit Anti-ULK1 Polyclonal Antibody (SPC-640) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1/2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~112.6kDa.)
WB (Western Blot)
(Western blot analysis of Rat Brain cell lysates showing detection of ~112.6kDa ULK1 protein using Rabbit Anti-ULK1 Polyclonal Antibody (SPC-640). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 2% GE Healthcare Blocker (RT, 60 minutes). Primary Antibody: Rabbit Anti-ULK1 Polyclonal Antibody (SPC-640) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1/2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~112.6kDa.)
WB (Western Blot)
(Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of 112.6 kDa ULK1 protein using Rabbit Anti-ULK1 Polyclonal Antibody (SPC-640). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ULK1 Polyclonal Antibody (SPC-640) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 112.6 kDa.)
WB (Western Blot)
(Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of 112.6 kDa ULK1 protein using Rabbit Anti-ULK1 Polyclonal Antibody (SPC-640). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ULK1 Polyclonal Antibody (SPC-640) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 112.6 kDa.)
Related Product Information for anti-ULK1 antibody
UNC-51 like kinase 1 (ULK1) is widely expresed and contains an amino-terminal kinase domain followed by a central proline-serine rich domain and a highly conserved carboxy-terminal domain. It has been linked to axon growth and is essential for autophagy. Structurally, ULK1 is similar to ATG1, and it appears that both Atg1/ULK1 can bind to several ATG proteins regulating phosphorylation states and protein trafficking.
Product Categories/Family for anti-ULK1 antibody
References
1. Okazaki N., et al. (2000) Brain Res Mol Brain Res. 85: 1-12. 2. Young A.R., et al. (2006) J Cell Sci. 119: 3888-900. 3. Kamada Y., et al. (2000) J Cell Biol. 150: 1507-13. 4. Lee S.B, et al. (2007) EMBO Rep. 8: 360-5. 5. Hara T., et al. (2008) J Cell Biol. 181: 497-510.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
112,631 Da
NCBI Official Full Name
serine/threonine-protein kinase ULK1
NCBI Official Synonym Full Names
unc-51 like autophagy activating kinase 1
NCBI Official Symbol
ULK1
NCBI Official Synonym Symbols
ATG1; ATG1A; UNC51; hATG1; Unc51.1
NCBI Protein Information
serine/threonine-protein kinase ULK1
UniProt Protein Name
Serine/threonine-protein kinase ULK1
UniProt Gene Name
ULK1
UniProt Synonym Gene Names
KIAA0722; ATG1; hATG1
Similar Products
Product Notes
The ULK1 ulk1 (Catalog #AAA104186) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ULK1 Antibody: ATTO 390 reacts with Human; Mouse; Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ULK1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ULK1 ulk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ULK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.