IF (Immunofluorescence)
(AAA329094 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA329094 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Rabbit URP2 Polyclonal Antibody | anti-FERMT3 antibody
URP2 Antibody
Gene Names
FERMT3; URP2; KIND3; MIG-2; MIG2B; URP2SF; UNC112C
Reactivity
Human, MousePredicted Reactivity: Pig(88%), Bovine(88%), Horse(88%), Sheep(88%), Dog(88%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
URP2, Antibody; URP2 Antibody; Fermitin family homolog 3; Fermitin family member 3; FERMT3; Kind3; Kindlin 3; Kindlin-3; Kindlin3; MGC10966; MIG 2; MIG2 like protein; MIG2-like protein; MIG2B; Unc 112 related protein 2; Unc-112-related protein 2; UNC112C; URP2; URP2_HUMAN; URP2SF; anti-FERMT3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Predicted Reactivity: Pig(88%), Bovine(88%), Horse(88%), Sheep(88%), Dog(88%)
Predicted Reactivity: Pig(88%), Bovine(88%), Horse(88%), Sheep(88%), Dog(88%)
Clonality
Polyclonal
Isotype
IgG
Specificity
URP2 Antibody detects endogenous levels of total URP2.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
667
Applicable Applications for anti-FERMT3 antibody
ELISA
Immunogen
A synthesized peptide derived from human URP2, corresponding to a region within the internal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA329094 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA329094 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
IF (Immunofluorescence)
(AAA329094 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA329094 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
IHC (Immunohiostchemistry)
(AAA329094 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(AAA329094 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from Human lung, using URP2 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Human lung, using URP2 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-FERMT3 antibody
Function: Plays a central role in cell adhesion in hematopoietic cells (PubMed:19234463, PubMed:26359933). Acts by activating the integrin beta-1-3 (ITGB1, ITGB2 and ITGB3) (By similarity). Required for integrin-mediated platelet adhesion and leukocyte adhesion to endothelial cells (PubMed:19234460). Required for activation of integrin beta-2 (ITGB2) in polymorphonuclear granulocytes (PMNs) (By similarity).Isoform 2 may act as a repressor of NF-kappa-B and apoptosis.
Subcellular Location: Cell projection>Podosome. Note: Present in the F-actin surrounding ring structure of podosomes, which are specialized adhesion structures of hematopoietic cells.
Tissue Specificity: Highly expressed in lymph node. Expressed in thymus, spleen and leukocytes. Weakly expressed in placenta, small intestine, stomach, testis and lung. Overexpressed in B-cell malignancies.
Subunit Structure: Interacts with ITGB1, ITGB2 and ITGB3 (via cytoplasmic tails).
Similarity: The FERM domain is not correctly detected by PROSITE or Pfam techniques because it contains the insertion of a PH domain.Belongs to the kindlin family.
Subcellular Location: Cell projection>Podosome. Note: Present in the F-actin surrounding ring structure of podosomes, which are specialized adhesion structures of hematopoietic cells.
Tissue Specificity: Highly expressed in lymph node. Expressed in thymus, spleen and leukocytes. Weakly expressed in placenta, small intestine, stomach, testis and lung. Overexpressed in B-cell malignancies.
Subunit Structure: Interacts with ITGB1, ITGB2 and ITGB3 (via cytoplasmic tails).
Similarity: The FERM domain is not correctly detected by PROSITE or Pfam techniques because it contains the insertion of a PH domain.Belongs to the kindlin family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)75kD.
Predicted Molecular Weight: (Calculated)76kDa.
Predicted Molecular Weight: (Calculated)76kDa.
NCBI Official Full Name
fermitin family homolog 3 long isoform
NCBI Official Synonym Full Names
fermitin family member 3
NCBI Official Symbol
FERMT3
NCBI Official Synonym Symbols
URP2; KIND3; MIG-2; MIG2B; URP2SF; UNC112C
NCBI Protein Information
fermitin family homolog 3
UniProt Protein Name
Fermitin family homolog 3
UniProt Gene Name
FERMT3
UniProt Synonym Gene Names
KIND3; MIG2B; URP2
UniProt Entry Name
URP2_HUMAN
Similar Products
Product Notes
The FERMT3 fermt3 (Catalog #AAA329094) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The URP2 Antibody reacts with Human, Mouse Predicted Reactivity: Pig(88%), Bovine(88%), Horse(88%), Sheep(88%), Dog(88%) and may cross-react with other species as described in the data sheet. AAA Biotech's URP2 can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the FERMT3 fermt3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "URP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.