IF (Immunofluorescence)
(Figure 2. IF analysis of Ush2a using anti-Ush2a antibody (AAA126669).Ush2a was detected in cryosections of Ush2a mutant zebrafish retina tissue without prior fixation. The tissue section was blocked with Blocking buffer (10% NGS, 2% BSA, 0.1% Tween in PBS) for 30 minutes. The tissue section was then incubated with rabbit anti-Ush2a Antibody (AAA126669) in blocking solution at 1:200 dilution overnight at 4 degree C. Alexa Fluor 568 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody a and incubated for 1 hour at room temperature in blocking solution. Post immuno-fixation with 4% PFA in PBS for 10 minutes. Mount in Prolong gold.)
Rabbit anti-Zebrafish Ush2a Polyclonal Antibody | anti-USH2A antibody
Anti-Zebrafish Ush2a Antibody
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
IF (Immunofluorescence)
(Figure 2. IF analysis of Ush2a using anti-Ush2a antibody (AAA126669).Ush2a was detected in cryosections of Ush2a mutant zebrafish retina tissue without prior fixation. The tissue section was blocked with Blocking buffer (10% NGS, 2% BSA, 0.1% Tween in PBS) for 30 minutes. The tissue section was then incubated with rabbit anti-Ush2a Antibody (AAA126669) in blocking solution at 1:200 dilution overnight at 4 degree C. Alexa Fluor 568 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody a and incubated for 1 hour at room temperature in blocking solution. Post immuno-fixation with 4% PFA in PBS for 10 minutes. Mount in Prolong gold.)
IF (Immunofluorescence)
(Figure 2. IF analysis of Ush2a using anti-Ush2a antibody (AAA126669).Ush2a was detected in cryosections of Ush2a mutant zebrafish retina tissue without prior fixation. The tissue section was blocked with Blocking buffer (10% NGS, 2% BSA, 0.1% Tween in PBS) for 30 minutes. The tissue section was then incubated with rabbit anti-Ush2a Antibody (AAA126669) in blocking solution at 1:200 dilution overnight at 4 degree C. Alexa Fluor 568 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody a and incubated for 1 hour at room temperature in blocking solution. Post immuno-fixation with 4% PFA in PBS for 10 minutes. Mount in Prolong gold.)
IF (Immunofluorescence)
(Figure 1. IF analysis of Ush2a using anti-Ush2a antibody (AAA126669).Ush2a was detected in cryosections of wildtype zebrafish retina tissue without prior fixation. The tissue section was blocked with Blocking buffer (10% NGS, 2% BSA, 0.1% Tween in PBS) for 30 minutes. The tissue section was then incubated with rabbit anti-Ush2a Antibody (AAA126669) in blocking solution at 1:200 dilution overnight at 4 degree C. Alexa Fluor 568 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody a and incubated for 1 hour at room temperature in blocking solution. Post immuno-fixation with 4% PFA in PBS for 10 minutes. Mount in Prolong gold.)
IF (Immunofluorescence)
(Figure 1. IF analysis of Ush2a using anti-Ush2a antibody (AAA126669).Ush2a was detected in cryosections of wildtype zebrafish retina tissue without prior fixation. The tissue section was blocked with Blocking buffer (10% NGS, 2% BSA, 0.1% Tween in PBS) for 30 minutes. The tissue section was then incubated with rabbit anti-Ush2a Antibody (AAA126669) in blocking solution at 1:200 dilution overnight at 4 degree C. Alexa Fluor 568 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody a and incubated for 1 hour at room temperature in blocking solution. Post immuno-fixation with 4% PFA in PBS for 10 minutes. Mount in Prolong gold.)
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Product Notes
The USH2A (Catalog #AAA126669) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Zebrafish Ush2a Antibody reacts with Zebrafish and may cross-react with other species as described in the data sheet. It is sometimes possible for the material contained within the vial of "Ush2a, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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