IP (Immunoprecipitation)
(Detection of human USP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP1 antibody (AAA211594 lot 3) used for IP at 6 ug per reaction. USP1 was also immunoprecipitated by a previous lot of this antibody (AAA211594 lot 2) and a second antibody against a different epitope of USP1 . For blotting immunoprecipitated USP1, AAA211594 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human USP1 Polyclonal Antibody | anti-USP1 antibody
Rabbit anti-USP1 Antibody, Affinity Purified
Reactivity
Human
Applications
Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
USP1, Antibody; Rabbit anti-USP1 Antibody, Affinity Purified; ubiquitin specific processing protease 1; Ubiquitin-specific-processing protease 1; ubiquitin thiolesterase 1; ubiquitin specific protease 1; ubiquitin carboxyl-terminal hydrolase 1; hUBP; deubiquitinating enzyme 1; UBP; ubiquitin thioesterase 1; Ubiquitin carboxyl-terminal hydrolase 1; ubiquitin specific peptidase 1; anti-USP1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-USP1 antibody
IP (Immunoprecipitation)
Predicted Reactivity
Mouse, Rat, Bovine
Immunogen
Between 450 and 500
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human USP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP1 antibody (AAA211594 lot 3) used for IP at 6 ug per reaction. USP1 was also immunoprecipitated by a previous lot of this antibody (AAA211594 lot 2) and a second antibody against a different epitope of USP1 . For blotting immunoprecipitated USP1, AAA211594 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human USP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP1 antibody (AAA211594 lot 3) used for IP at 6 ug per reaction. USP1 was also immunoprecipitated by a previous lot of this antibody (AAA211594 lot 2) and a second antibody against a different epitope of USP1 . For blotting immunoprecipitated USP1, AAA211594 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human USP1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-USP1 (Cat. No. AAA211594) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human USP1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-USP1 (Cat. No. AAA211594) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-USP1 antibody
Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 1 (USP1) has been found to play a role in the Fanconi anemia DNA repair pathway. USP1 specifically removes monoubiquitination from the Fanconi anemia D2 (FANCD2) protein and from the proliferating cell nuclear antigen (PCNA). Deubiquitination of FANCD2 and PCNA is critical to their role in the DNA damage response and DNA crosslink repair.
Product Categories/Family for anti-USP1 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The USP1 usp1 (Catalog #AAA211594) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-USP1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's USP1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation). Researchers should empirically determine the suitability of the USP1 usp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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