Rabbit anti-Human, Rat USP10 Polyclonal Antibody | anti-USP10 antibody

USP10 Antibody

Cat. Num. AAA233992

• Gene Names: USP10; UBPO
• Reactivity: Human, Rat
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
• Purity: >95%, Protein G purified
• Synonyms: USP10, Antibody; USP10 Antibody; Ubiquitin carboxyl-terminal hydrolase 10; Deubiquitinating enzyme 10; Ubiquitin thioesterase 10; Ubiquitin-specific-processing protease 10; USP10; KIAA0190; anti-USP10 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 802

• Applicable Applications for anti-USP10 antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Immunogen: Recombinant Human Ubiquitin carboxyl-terminal hydrolase 10 protein (453-592AA)
• Conjugation: Non-conjugated
• Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

IHC (Immunohistochemisry)

(Immunofluorescent analysis of Hela cells using AAA233992 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

IHC (Immunohiostchemistry)

(Immunohistochemistry of paraffin-embedded human testis tissue using AAA233992 at dilution of 1:100)

WB (Western Blot)

(Western BlotPositive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, Rat liver tissueAll lanes: USP10 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 88, 93 KDaObserved band size: 88 KDa)

Related Product Information for anti-USP10 antibody

Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, BECN1, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling. Involved in a TANK-dependent negative feedback response to attenuate NF-kappaB activation via deubiquitinating IKBKG or TRAF6 in response to interleukin-1-beta (IL1B) stimulation or upon DNA damage (PubMed:25861989).

Product Categories/Family for anti-USP10 antibody

Epigenetics and Nuclear Signaling; Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 440918695
• NCBI GeneID: 9100
• NCBI Accession #: NP_001259004.1
• NCBI GenBank Nucleotide #: NM_001272075.1
• UniProt Accession #: Q14694; Q9BWG7; Q9NSL7; B2RDJ8; B4DS84
• Molecular Weight: 87,533 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 10 isoform 1
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 10
• NCBI Official Symbol: USP10
• NCBI Official Synonym Symbols: UBPO
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 10
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 10
• UniProt Gene Name: USP10
• UniProt Synonym Gene Names: KIAA0190