Rabbit anti-Human, rat USP2 Polyclonal Antibody | anti-USP2 antibody

USP2 Antibody (N-term)

Cat. Num. AAA285647

• Gene Names: USP2; USP9; UBP41
• Reactivity: Human, rat
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: USP2, Antibody; USP2 Antibody (N-term); Ubiquitin carboxyl-terminal hydrolase 2; 41 kDa ubiquitin-specific protease; Deubiquitinating enzyme 2; Ubiquitin thioesterase 2; Ubiquitin-specific-processing protease 2; USP2; UBP41; anti-USP2 antibody

• Host: Rabbit
• Reactivity: Human, rat
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This USP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-101 amino acids from the N-terminal region of human USP2.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 71-101
• Sequence Length: 362

• Applicable Applications for anti-USP2 antibody: IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(Western blot analysis of USP2 (arrow) using rabbit polyclonal USP2 Antibody (T22). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the USP2 gene.)

IHC (Immunohistochemisry)

(IHC for USP2a in a formalin-fixed, paraffin-embedded human prostate sample. Cytoplasmic positive immunostaining was detected in tumor glands (ii), whereas in normal prostatic epithelium, USP2a expression was restricted to the basal layer.)

WB (Western Blot)

(Western blot analysis of USP2 Antibody (N-term) in HL60 cell line lysates (35ug/lane). USP2 (arrow) was detected using the purified Pab.)

WB (Western Blot)

(Western blot analysis of lysate from R. heart cell line, using USP2 Antibody (T22). AAA285647 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)

Related Product Information for anti-USP2 antibody

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Product Categories/Family for anti-USP2 antibody

Cell Biology

References

Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 344313155
• NCBI GeneID: 9099
• NCBI Accession #: NP_001230688.1; NP_004196.4; NP_741994.1
• NCBI GenBank Nucleotide #: NM_001243759.1
• UniProt Accession #: O75604; Q8IUM2; Q8IW04; Q96MB9; Q9BQ21; B0YJB8; E9PPM2
• Molecular Weight: 68072
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 2 isoform c
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 2
• NCBI Official Symbol: USP2
• NCBI Official Synonym Symbols: USP9; UBP41
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 2
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 2
• UniProt Gene Name: USP2
• UniProt Synonym Gene Names: UBP41
• UniProt Entry Name: UBP2_HUMAN