WB (Western Blot)
(Detection of human USP20/VDU2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-USP20/VDU2 antibody AAA211348 (lot AAA211348-2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. USP20/VDU2 was also immunoprecipitated by a previous lot (lot AAA211348-1) of this antibody. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)
Rabbit anti-Human, Mouse USP20/VDU2 Polyclonal Antibody | anti-USP20/VDU2 antibody
Rabbit anti-USP20/VDU2 Antibody, Affinity Purified
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
USP20/VDU2, Antibody; Rabbit anti-USP20/VDU2 Antibody, Affinity Purified; ubiquitin carboxyl-terminal hydrolase 20; VDU2; ubiquitin-specific-processing protease 20; VHL-interacting deubiquitinating enzyme 2; ubiquitin thioesterase 20; LSFR3A; hVDU2; deubiquitinating enzyme 20; ubiquitin thiolesterase 20; pVHL-interacting deubiquitinating enzyme 2; Ubiquitin carboxyl-terminal hydrolase 20; ubiquitin specific peptidase 20; anti-USP20/VDU2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-USP20/VDU2 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 864 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human USP20/VDU2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-USP20/VDU2 antibody AAA211348 (lot AAA211348-2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. USP20/VDU2 was also immunoprecipitated by a previous lot (lot AAA211348-1) of this antibody. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)
WB (Western Blot)
(Detection of human USP20/VDU2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-USP20/VDU2 antibody AAA211348 (lot AAA211348-2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. USP20/VDU2 was also immunoprecipitated by a previous lot (lot AAA211348-1) of this antibody. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)
WB (Western Blot)
(Detection of mouse USP20/VDU2 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-USP20/VDU2 antibody AAA211348 (lot AAA211348-2) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of mouse USP20/VDU2 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-USP20/VDU2 antibody AAA211348 (lot AAA211348-2) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-USP20/VDU2 antibody
The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific peptidase 20 (USP20) is a UBP and is also called von-Hippel-Lindau protein (pVHL) interacting deubiquitinating enzyme 2 (VDU2). pVHL is an E3 ubiquitin-ligase that ubiquitinates hypoxia-inducible factor (HIF)-1alpha. USP20/VDU2 functions to specifically deubiquitinate HIF-1alpha to increase expression of HIF-1alpha regulated genes.
Product Categories/Family for anti-USP20/VDU2 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The USP20/VDU2 usp20 (Catalog #AAA211348) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-USP20/VDU2 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's USP20/VDU2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the USP20/VDU2 usp20 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP20/VDU2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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