Rabbit anti-Human, Mouse USP25 Polyclonal Antibody | anti-USP25 antibody

USP25 Antibody

Cat. Num. AAA40550

• Gene Names: USP25; USP21
• Reactivity: Human, Mouse
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
• Purity: USP25 antibody is affinity chromatography purified via peptide column.
• Synonyms: USP25, Antibody; USP25 Antibody; USP25; USP21; Ubiquitin carboxyl-terminal hydrolase 25; Deubiquitinating enzyme 25; ubiquitin specific peptidase 25; anti-USP25 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: USP25 antibody is human and mouse reactive. Multiple isoforms of USP25 are known to exist.
• Purity/Purification: USP25 antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 1055

• Applicable Applications for anti-USP25 antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Conjugate: Unconjugated
• Immunogen: USP25 antibody was raised against a 17 amino acid peptide near the center of human USP25.
• Buffer: USP25 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: USP25 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

IF (Immunofluorescence)

(Immunofluorescence of USP25 in human brain tissue with USP25 antibody at 20 μg/mL.)

IHC (Immunohiostchemistry)

(Immunohistochemistry of USP25 in human brain tissue with USP25 antibody at 5 μg/mL.)

WB (Western Blot)

(Western blot analysis of USP25 in mouse brain tissue lysate with USP25 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)

Related Product Information for anti-USP25 antibody

USP25 (ubiquitin specific peptidase 25), also known as USP21, belongs to the peptidase C19 family and is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response (1). It contains one UBA-like domain and two UIM (ubiquitin-interacting motif) repeats. Due to alternative splicing events, USP25 is expressed as two short, ubiquitously expressed isoforms and one long, muscle-specific isoform (2). The long isoform of USP25 (USP25m) is upregulated in myogenesis and is implicated in regulation of muscular differentiation and function. USP25 is a deubiquitinating enzyme (DUB) that negatively regulates IL-17-triggered signaling (3,4).

NCBI and Uniprot Product Information

• NCBI GI #: 50312666
• NCBI GeneID: 29761
• NCBI Accession #: NP_037528
• NCBI GenBank Nucleotide #: NM_013396.4
• UniProt Accession #: Q9UHP3; Q6DHZ9; Q9H9W1; C0LSZ0
• Molecular Weight: Predicted: 116 kDa; ; Observed: 105 kDa
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 25 isoform USP25a
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 25
• NCBI Official Symbol: USP25
• NCBI Official Synonym Symbols: USP21
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 25; USP on chromosome 21; ubiquitin thioesterase 25; deubiquitinating enzyme 25; ubiquitin thiolesterase 25; ubiquitin-specific processing protease 25
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 25
• UniProt Gene Name: USP25
• UniProt Synonym Gene Names: USP21
• UniProt Entry Name: UBP25_HUMAN