IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human ovarian cancer using AAA231091 at dilution of 1:100)
Rabbit anti-Human USP28 Polyclonal Antibody | anti-USP28 antibody
USP28 Antibody
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Antigen Affinity Purified
Synonyms
USP28, Antibody; USP28 Antibody; Ubiquitin carboxyl-terminal hydrolase 28; Deubiquitinating enzyme 28; Ubiquitin thioesterase 28; Ubiquitin-specific-processing protease 28; USP28; KIAA1515; anti-USP28 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid
Sequence Length
1077
Applicable Applications for anti-USP28 antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA
Species
Human
Immunogen
Recombinant human Ubiquitin carboxyl-terminal hydrolase 28 protein (430-580AA)
Conjugate
Non-conjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-79312
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human ovarian cancer using AAA231091 at dilution of 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human ovarian cancer using AAA231091 at dilution of 1:100)
WB (Western Blot)
(Western blotAll lanes: USP28 antibody at 3.08 ug/ml+ Hela whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 123,120,67 kDaObserved band size: 122,50 kDa)
WB (Western Blot)
(Western blotAll lanes: USP28 antibody at 3.08 ug/ml+ Hela whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 123,120,67 kDaObserved band size: 122,50 kDa)
Related Product Information for anti-USP28 antibody
Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in a subset of colorectal cancers (CRC) cells (PubMed:24623306).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
66,243 Da
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase 28 isoform 1
NCBI Official Synonym Full Names
ubiquitin specific peptidase 28
NCBI Official Symbol
USP28
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase 28
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase 28
UniProt Gene Name
USP28
UniProt Synonym Gene Names
KIAA1515
UniProt Entry Name
UBP28_HUMAN
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Product Notes
The USP28 usp28 (Catalog #AAA231091) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The USP28 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's USP28 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the USP28 usp28 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP28, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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