IP (Immunoprecipitation)
(Detection of human USP47 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-USP47 antibody AAA211295 used for IP at 3 ug/mg lysate. USP47 was also immunoprecipitated by rabbit anti-USP47 antibody which recognizes an upstream epitope. For blotting immunoprecipitated USP47, was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Rabbit anti-Human USP47 Polyclonal Antibody | anti-USP47 antibody
Rabbit anti-USP47 Antibody, Affinity Purified
Gene Names
USP47; TRFP; FLJ20727; DKFZp686C13257
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
USP47, Antibody; Rabbit anti-USP47 Antibody, Affinity Purified; ubiquitin thioesterase 47; ubiquitin-specific-processing protease 47; ubiquitin thiolesterase 47; TRFP; Trf (TATA binding protein-related factor)-proximal homolog; deubiquitinating enzyme 47; ubiquitin carboxyl-terminal hydrolase 47; Ubiquitin carboxyl-terminal hydrolase 47; ubiquitin specific peptidase 47; anti-USP47 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-USP47 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Predicted Reactivity
Chicken
Immunogen
Between 1325 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human USP47 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-USP47 antibody AAA211295 used for IP at 3 ug/mg lysate. USP47 was also immunoprecipitated by rabbit anti-USP47 antibody which recognizes an upstream epitope. For blotting immunoprecipitated USP47, was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human USP47 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-USP47 antibody AAA211295 used for IP at 3 ug/mg lysate. USP47 was also immunoprecipitated by rabbit anti-USP47 antibody which recognizes an upstream epitope. For blotting immunoprecipitated USP47, was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistochemistry)
(Detection of human USP47 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-USP47 (Cat. No. AAA211295 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human USP47 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-USP47 (Cat. No. AAA211295 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-USP47 antibody
Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific peptidase (USP47) is a UBP.
Product Categories/Family for anti-USP47 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
7,160 Da
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase 47
NCBI Official Synonym Full Names
ubiquitin specific peptidase 47
NCBI Official Symbol
USP47
NCBI Official Synonym Symbols
TRFP; FLJ20727; DKFZp686C13257
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase 47; OTTHUMP00000230750; OTTHUMP00000230781; OTTHUMP00000230782; ubiquitin thioesterase 47; deubiquitinating enzyme 47; ubiquitin thiolesterase 47; ubiquitin specific protease 47; ubiquitin-specific-processing protease
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase 47
UniProt Gene Name
USP47
UniProt Entry Name
UBP47_HUMAN
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Product Notes
The USP47 usp47 (Catalog #AAA211295) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-USP47 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's USP47 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the USP47 usp47 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP47, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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