WB (Western Blot)
(Detection of mouse USP48 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP48 antibody (AAA211349 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Rabbit anti-Human, Mouse USP48 Polyclonal Antibody | anti-USP48 antibody
Rabbit anti-USP48 Antibody, Affinity Purified
Gene Names
USP48; USP31; RAP1GA1
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
USP48, Antibody; Rabbit anti-USP48 Antibody, Affinity Purified; deubiquitinating enzyme 48; RAP1GA1; ubiquitin carboxyl-terminal hydrolase 48; ubiquitin specific protease 31; ubiquitin thioesterase 48; ubiquitin thiolesterase 48; Ubiquitin-specific peptidase 48; Ubiquitin-specific protease 48; ubiquitin-specific-processing protease 48; USP31; Ubiquitin carboxyl-terminal hydrolase 48; ubiquitin specific peptidase 48; anti-USP48 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-USP48 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, Chicken
Immunogen
Between 985 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse USP48 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP48 antibody (AAA211349 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of mouse USP48 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP48 antibody (AAA211349 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of human USP48 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, RKO, GaMG, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP48 antibody (AAA211349 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human USP48 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, RKO, GaMG, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP48 antibody (AAA211349 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human USP48 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP48 antibody (AAA211349 lot 3) used for IP at 6 ug per reaction. USP48 was also immunoprecipitated by a previous lot of this antibody (AAA211349 lot 2). For blotting immunoprecipitated USP48, AAA211349 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human USP48 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP48 antibody (AAA211349 lot 3) used for IP at 6 ug per reaction. USP48 was also immunoprecipitated by a previous lot of this antibody (AAA211349 lot 2). For blotting immunoprecipitated USP48, AAA211349 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Related Product Information for anti-USP48 antibody
The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific peptidase 48 (USP48) is a member of the C19 peptidase family involved in the processing of polyubiquitin precursors as well as that of ubiquitinated proteins. USP48 is involved in the activation of NF-kappa B via its interaction with RELA and TRAF2.
Product Categories/Family for anti-USP48 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
120,526 Da
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase 48 isoform a
NCBI Official Synonym Full Names
ubiquitin specific peptidase 48
NCBI Official Symbol
USP48
NCBI Official Synonym Symbols
USP31; RAP1GA1
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase 48; ubiquitin thioesterase 48; deubiquitinating enzyme 48; ubiquitin thiolesterase 48; ubiquitin specific protease 31; ubiquitin specific protease 48; ubiquitin-specific-processing protease 48
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase 48
UniProt Gene Name
USP48
UniProt Synonym Gene Names
USP31
UniProt Entry Name
UBP48_HUMAN
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Product Notes
The USP48 usp48 (Catalog #AAA211349) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-USP48 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's USP48 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the USP48 usp48 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP48, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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