Rabbit UXT Polyclonal Antibody | anti-UXT antibody

UXT Antibody

Cat. Num. AAA329188

• Gene Names: UXT; STAP1; ART-27
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: UXT, Antibody; UXT Antibody; Androgen receptor trapped clone 27 protein; ART 27; ART-27; OTTHUMP00000023226; OTTHUMP00000023227; Protein UXT; SKP2 associated alpha PFD 1; STAP1; Ubiquitously expressed prefoldin like chaperone; Ubiquitously expressed transcript; Ubiquitously expressed transcript protein; Uxt; UXT_HUMAN; anti-UXT antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: UXT Antibody detects endogenous levels of total UXT.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 157

• Applicable Applications for anti-UXT antibody: ELISA
• Immunogen: A synthesized peptide derived from human UXT, corresponding to a region within the internal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

IHC (Immunohistochemistry)

(AAA329188 at 1/100 staining Mouse colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemisry)

(AAA329188 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohiostchemistry)

(AAA329188 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot)

(Western blot analysis of extracts from Human adrenal gland, using UXT Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-UXT antibody

Function: Involved in gene transcription regulation (PubMed:28106301, PubMed:21730289). Acts in concert with the corepressor URI1 to regulate androgen receptor AR-mediated transcription (PubMed:11854421, PubMed:21730289). Together with URI1, associates with chromatin to the NKX3-1 promoter region (PubMed:21730289). Negatively regulates the transcriptional activity of the estrogen receptor ESR1 by inducing its translocation into the cytoplasm (PubMed:28106301). May act as nuclear chaperone that facilitates the formation of the NF-kappa-B enhanceosome and thus positively regulates NF-kappa-B transcription activity (PubMed:17620405, PubMed:21307340). Potential component of mitochondrial-associated LRPPRC, a multidomain organizer that potentially integrates mitochondria and the microtubular cytoskeleton with chromosome remodeling (PubMed:17554592). Increasing concentrations of UXT contributes to progressive aggregation of mitochondria and cell death potentially through its association with LRPPRC (PubMed:17554592). Suppresses cell transformation and it might mediate this function by interaction and inhibition of the biological activity of cell proliferation and survival stimulatory factors like MECOM (PubMed:17635584).Plays a role in protecting cells against TNF-alpha-induced apoptosis by preventing the recruitment of FADD and caspase 8 to the apoptotic complex I, composed of TRADD, TRAF2 and RIPK1/RIP.

Subcellular Location: Cytoplasm. Cytoplasm. Nucleus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Spindle pole. Note: Predominantly localizes to the nucleus (PubMed:16221885). Localizes to spindle pole during mitosis (PubMed:16221885).

Tissue Specificity: Ubiquitous (PubMed:10087202, PubMed:11854421, PubMed:17635584, PubMed:11827465). Expressed in prostate epithelial cells (PubMed:21730289). Expressed in mammary epithelial cells (PubMed:28106301). Highest levels in the heart, skeletal muscle, pancreas, kidney, liver, adrenal gland, peripheral blood leukocytes, lymph node, prostate, and thyroid and the lowest levels in bladder and uterus (PubMed:11854421, PubMed:17635584, PubMed:11827465). Overexpressed in a number of tumor tissues (PubMed:11854421, PubMed:16221885, PubMed:28106301).

Subunit Structure: Homohexamer (PubMed:16221885). Interacts with LRPPRC (PubMed:11827465, PubMed:17554592). Interacts with androgen receptor AR (via N-terminus) (PubMed:11854421). Interacts with estrogen receptor ESR1; the interaction relocalizes ESR1 from the nucleus to the cytoplasm (PubMed:28106301). In the nucleus, interacts specifically with RELA (via RHD domain) and forms a dynamic complex with NF-kappa-B and is recruited to the NF-kappa-B enhanceosome upon stimulation (PubMed:17620405). Interacts with MECOM (PubMed:17635584). Interacts with URI1 (PubMed:21730289). Isoform 1 is part of complex I composed of TNF-alpha receptor TNFRSF1A, TRADD, TRAF2 and RIPK1 formed in response to TNF-alpha stimulation. Within the complex, interacts (via TPQE motif) with TRAF2; the interaction prevents the recruitment of FADD and CASP8/caspase 8 to complex I (PubMed:21307340).

Similarity: Belongs to the UXT family.

NCBI and Uniprot Product Information

• NCBI GI #: 8928445
• NCBI GeneID: 8409
• UniProt Accession #: Q9UBK9
• Molecular Weight: Observed Molecular Weight: (Observed)18kD.; Predicted Molecular Weight: (Calculated)18kDa.
• NCBI Official Full Name: Protein UXT
• NCBI Official Synonym Full Names: ubiquitously expressed prefoldin like chaperone
• NCBI Official Symbol: UXT
• NCBI Official Synonym Symbols: STAP1; ART-27
• NCBI Protein Information: protein UXT
• UniProt Protein Name: Protein UXT
• UniProt Gene Name: UXT
• UniProt Synonym Gene Names: ART-27
• UniProt Entry Name: UXT_HUMAN