WB (Western Blot)
(Western blot analysis of V-ATPase H using HuvEc whole cell lysates)
Rabbit anti-Human, Mouse V-ATPase H Polyclonal Antibody | anti-ATP6V1H antibody
V-ATPase H Antibody
Gene Names
ATP6V1H; SFD; NBP1; VMA13; CGI-11; MSTP042; SFDbeta; SFDalpha
Reactivity
Human, Mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
V-ATPase H, Antibody; V-ATPase H Antibody; Atp6v1h; ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H; CGI 11; CGI11; EC 3.6.3.14; MSTP042; NBP1; Nef binding protein 1; Nef-binding protein 1; Protein VMA13 homolog; SFD; SFDalpha; SFDbeta; V ATPase subunit H; V-ATPase 50/57 kDa subunits; V-ATPase subunit H; V-type proton ATPase subunit H; Vacuolar ATP synthase subunit H; Vacuolar proton pump subunit H; Vacuolar proton pump subunit SFD; VATH_HUMAN; VMA13; anti-ATP6V1H antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
V-ATPase H antibody detects endogenous levels of total V-ATPase H
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
483
Applicable Applications for anti-ATP6V1H antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human V-ATPase H
Tissue Specificity
Widely expressed.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of V-ATPase H using HuvEc whole cell lysates)
WB (Western Blot)
(Western blot analysis of V-ATPase H using HuvEc whole cell lysates)
IHC (Immunohistochemistry)
(AAA322649 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA322649 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-ATP6V1H antibody
Function: Subunit of the peripheral V1 complex of vacuolar ATPase. Subunit H activates the ATPase activity of the enzyme and couples ATPase activity to proton flow. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system (By similarity). Involved in the endocytosis mediated by clathrin-coated pits, required for the formation of endosomes.
Subunit Structure: V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d). Interacts with HIV-1 Nef protein (PubMed:12032142, PubMed:9620685). Interacts with AP2M1 (PubMed:12032142). Interacts with TM9SF4 in colon cancer cells (PubMed:25659576).
Similarity: Belongs to the V-ATPase H subunit family.
Subunit Structure: V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d). Interacts with HIV-1 Nef protein (PubMed:12032142, PubMed:9620685). Interacts with AP2M1 (PubMed:12032142). Interacts with TM9SF4 in colon cancer cells (PubMed:25659576).
Similarity: Belongs to the V-ATPase H subunit family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 55 kDa
Predicted: 56 kDa
Predicted: 56 kDa
NCBI Official Full Name
V-type proton ATPase subunit H isoform 1
NCBI Official Synonym Full Names
ATPase H+ transporting V1 subunit H
NCBI Official Symbol
ATP6V1H
NCBI Official Synonym Symbols
SFD; NBP1; VMA13; CGI-11; MSTP042; SFDbeta; SFDalpha
NCBI Protein Information
V-type proton ATPase subunit H
UniProt Protein Name
V-type proton ATPase subunit H
UniProt Gene Name
ATP6V1H
UniProt Synonym Gene Names
V-ATPase subunit H; NBP1
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Product Notes
The ATP6V1H atp6v1h (Catalog #AAA322649) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The V-ATPase H Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's V-ATPase H can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ATP6V1H atp6v1h for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "V-ATPase H, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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