Rabbit anti-Human VAPB Polyclonal Antibody | anti-VAPB antibody

Anti-VAPB Antibody, Rabbit Polyclonal

Cat. Num. AAA255146

• Gene Names: VAPB; ALS8; VAP-B; VAMP-B
• Reactivity: Human
• Applications: Immunoprecipitation, Immunohistochemistry, ELISA, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: VAPB, Antibody; Anti-VAPB Antibody, Rabbit Polyclonal; VAPB/VAP-B Antibody, Rabbit PAb, Antigen Affinity Purified; VAMP (vesicle-associated membrane protein)-associated protein B and C; Anti-ALS8 Antibody; Anti-VAMP-B Antibody; Anti-VAP-B Antibody; anti-VAPB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human VAPB/VAP-B
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-VAPB antibody: IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Immunogen: Recombinant Human VAPB/VAP-B protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human VAPB/VAP-B (rh VAPB/VAP-B). VAPB/VAP-B specific IgG was purified by Human VAPB/VAP-B affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

WB (Western Blot)

(Anti-VAPB rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: A549 Whole Cell LysateLysates/proteins at 60 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:27 kDaObserved band size:30 kDa)

IP (Immunoprecipitation)

(VAPB was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell LysateLane C:0.5 mg A549 Whole Cell Lysate2 uL anti-VAPB rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-VAPB rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 27 kDaObserved band size: 27 kDa)

IHC (Immunohiostchemistry)

(Immunochemical staining of human VAPB in human brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

IHC (Immunohistochemistry)

(Immunochemical staining of human VAPB in human liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-VAPB antibody

Vesicle-associated membrane protein-associated protein B/C, also known as VAMP-B/VAMP-C, VAMP-associated protein B/C, VAP-B/VAP-C and VAPB, is a single-pass type IV membrane protein that belongs to the VAMP-associated protein (VAP) family. VAPB contains one MSP domain. VAPB may play a role in vesicle trafficking. VAPB forms a heterodimer with VAPA. VAPB interacts with VAMP1 and VAMP2. Defects in VAPB are the cause of amyotrophic lateral sclerosis type 8 (ALS8) which is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Defects in VAPB are also a cause of spinal muscular atrophy autosomal dominant Finkel type (SMAF) which is characterized by proximal muscle weakness that begins in the lower limbs and then progresses to upper limbs.

References

Nishimura Y., et al., 1999, Biochem. Biophys. Res. Commun. 254:21-26. Gevaert K., et al., 2003, Nat. Biotechnol. 21:566-569. Hamamoto I., et al., 2005, J. Virol. 79:13473-13482. Choudhary C. et al., 2009, Science 325:834-840.

NCBI and Uniprot Product Information

• NCBI GI #: 307574674
• NCBI GeneID: 9217
• NCBI Accession #: NP_001182606.1
• NCBI GenBank Nucleotide #: NM_001195677.1
• UniProt Accession #: O95292; O95293; Q9P0H0; A2A2F2
• Molecular Weight: 11,152 Da
• NCBI Official Full Name: vesicle-associated membrane protein-associated protein B/C isoform 2
• NCBI Official Synonym Full Names: VAMP (vesicle-associated membrane protein)-associated protein B and C
• NCBI Official Symbol: VAPB
• NCBI Official Synonym Symbols: ALS8; VAP-B; VAMP-B
• NCBI Protein Information: vesicle-associated membrane protein-associated protein B/C; VAMP-associated 33 kDa protein
• UniProt Protein Name: Vesicle-associated membrane protein-associated protein B/C
• UniProt Gene Name: VAPB
• UniProt Synonym Gene Names: VAMP-B/VAMP-C; VAMP-associated protein B/C; VAP-B/VAP-C
• UniProt Entry Name: VAPB_HUMAN