FCM/FACS (Flow Cytometry)
(VDR Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human VDR Polyclonal Antibody | anti-VDR antibody
VDR Antibody (Center)
Gene Names
VDR; NR1I1; PPP1R163
Reactivity
Human
Applications
Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
VDR, Antibody; VDR Antibody (Center); Vitamin D3 receptor; VDR,25-dihydroxyvitamin D3 receptor; Nuclear receptor subfamily 1 group I member 1; VDR; NR1I1; anti-VDR antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This VDR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 274-299 amino acids from the Central region of human VDR.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
274-299
Sequence Length
477
Applicable Applications for anti-VDR antibody
WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(VDR Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(VDR Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Western blot analysis of lysates from PC-3, LNCaP cell line (from left to right), using VDR Antibody (Center). AAA288876 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of lysates from PC-3, LNCaP cell line (from left to right), using VDR Antibody (Center). AAA288876 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
Related Product Information for anti-VDR antibody
This gene encodes the nuclear hormone receptor for vitamin
D3. This receptor also functions as a receptor for the secondary
bile acid lithocholic acid. The receptor belongs to the family of
trans-acting transcriptional regulatory factors and shows sequence
similarity to the steroid and thyroid hormone receptors. Downstream
targets of this nuclear hormone receptor are principally involved
in mineral metabolism though the receptor regulates a variety of
other metabolic pathways, such as those involved in the immune
response and cancer. Mutations in this gene are associated with
type II vitamin D-resistant rickets. A single nucleotide
polymorphism in the initiation codon results in an alternate
translation start site three codons downstream. Alternative
splicing results in multiple transcript variants encoding the same
protein.
D3. This receptor also functions as a receptor for the secondary
bile acid lithocholic acid. The receptor belongs to the family of
trans-acting transcriptional regulatory factors and shows sequence
similarity to the steroid and thyroid hormone receptors. Downstream
targets of this nuclear hormone receptor are principally involved
in mineral metabolism though the receptor regulates a variety of
other metabolic pathways, such as those involved in the immune
response and cancer. Mutations in this gene are associated with
type II vitamin D-resistant rickets. A single nucleotide
polymorphism in the initiation codon results in an alternate
translation start site three codons downstream. Alternative
splicing results in multiple transcript variants encoding the same
protein.
Product Categories/Family for anti-VDR antibody
References
An, B.S., et al. Mol. Cell. Biol. 30(20):4890-4900(2010)
Elnenaei, M.O., et al. Br. J. Nutr., 1-8 (2010) In press :
Forghani, N., et al. J. Pediatr. Endocrinol. Metab. 23(8):843-850(2010)
Alvarez-Nava, F., et al. J. Pediatr. Endocrinol. Metab. 23(8):773-782(2010)
Jurutka, P.W., et al. Proc. Natl. Acad. Sci. U.S.A. 93(8):3519-3524(1996)
Elnenaei, M.O., et al. Br. J. Nutr., 1-8 (2010) In press :
Forghani, N., et al. J. Pediatr. Endocrinol. Metab. 23(8):843-850(2010)
Alvarez-Nava, F., et al. J. Pediatr. Endocrinol. Metab. 23(8):773-782(2010)
Jurutka, P.W., et al. Proc. Natl. Acad. Sci. U.S.A. 93(8):3519-3524(1996)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48289
NCBI Official Full Name
vitamin D3 receptor isoform VDRA
NCBI Official Synonym Full Names
vitamin D (1,25- dihydroxyvitamin D3) receptor
NCBI Official Symbol
VDR
NCBI Official Synonym Symbols
NR1I1; PPP1R163
NCBI Protein Information
vitamin D3 receptor
UniProt Protein Name
Vitamin D3 receptor
UniProt Gene Name
VDR
UniProt Synonym Gene Names
NR1I1; VDR
UniProt Entry Name
VDR_HUMAN
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Product Notes
The VDR vdr (Catalog #AAA288876) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 274-299. The VDR Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's VDR can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the VDR vdr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VDR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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