Rabbit anti-Human VEGFA Polyclonal Antibody | anti-VEGFA antibody

VEGFA Antibody

Cat. Num. AAA305651

• Gene Names: VEGFA; VPF; VEGF; MVCD1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Synonyms: VEGFA, Antibody; VEGFA Antibody; vascular endothelial growth factor A, VPF, VEGF, MVCD1; anti-VEGFA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total VEGFA protein.
• Form/Format: Supplied at 0.9mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.
• Sequence Length: 232

• Applicable Applications for anti-VEGFA antibody: IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Immunogen Type: Peptide
• Immunogen Description: Synthetic peptide corresponding to a region derived from 130-143 amino acids of human vascular endothelial growth factor A
• Target Name: VEGFA
• Preparation and Storage: Store at -20 degree C/1 year

Application Data

(Gel: 12%SDS-PAGELane1: Hela cell lysateLane2: 293T cell lysateLane3: HepG2 cell lysateLane4: HT29 cell lysateLane5: MDA231 cell lysateLane6: A549 cell lysateLane7: SKOV3 cell lysateLysates: 30 ug per lanePrimary antibody: 1/1000 dilutionSecondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilutionExposure time: 2 minutes)

IHC (Immunohiostchemistry)

(The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using 31274 (VEGFA Antibody) at dilution 1/25, on the right is treated with the synthetic peptide.)

Application Data

(Gel: 12%SDS-PAGELane1: Hela, cell lysateLane2: Jurkatcell lysateLane3: 293T cell lysateLane4: 231 cell lysateLysates: 40 ug per lanePrimary antibody: 1/450 dilutionSecondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilutionExposure time: 2 minutes)

Related Product Information for anti-VEGFA antibody

This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms.

Product Categories/Family for anti-VEGFA antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 284172459
• NCBI GeneID: 7422
• NCBI Accession #: NP_001165094
• NCBI GenBank Nucleotide #: NM_001171623.1
• UniProt Accession #: P15692; O60720; O75875; Q074Z4; Q16889; B5BU86; H0Y2S8; H0Y407; H0Y414; H0Y462; H0Y8N2; H3BLW7
• Molecular Weight: 34,406 Da
• NCBI Official Full Name: vascular endothelial growth factor A isoform i
• NCBI Official Synonym Full Names: vascular endothelial growth factor A
• NCBI Official Symbol: VEGFA
• NCBI Official Synonym Symbols: VPF; VEGF; MVCD1
• NCBI Protein Information: vascular endothelial growth factor A
• UniProt Protein Name: Vascular endothelial growth factor A
• UniProt Gene Name: VEGFA
• UniProt Synonym Gene Names: VEGF; VEGF-A; VPF
• UniProt Entry Name: VEGFA_HUMAN