WB (Western Blot)
(Left: Western blot image of HUVEC cells untreated (-) or treated with pervanadate (1mM) for 30 min. (+). Right: Western blot image of GST-recombinant VEGFR-2 kinase without (-) or with (+) akaline phosphatase treatment. Both sets of blots were probed with rabbit polyclonal anti-VEGFR-2 (a.a. 1304-1317) or anti-VEGFR-2 (Tyr-801).)
Rabbit VEGFR-2 Polyclonal Antibody | anti-VEGFR-2 antibody
VEGFR-2 (a.a.1304-1317) Antibody
Gene Names
KDR; FLK1; CD309; VEGFR; VEGFR2
Reactivity
Human, Rat, Mouse
Applications
Immunocytochemistry, ELISA, Western Blot
Synonyms
VEGFR-2, Antibody; VEGFR-2 (a.a.1304-1317) Antibody; VEGFR-2 (a.a.1304-1317); anti-VEGFR-2 antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Specificity
This antibody was affinity purified with VEGFR-2 (a.a. 1304-1317) peptide. The purified antibody detects a 180kDa* band corresponding to VEGFR-2 in western blots of human endothelial cells, and shows strong reactivity toward recombinant human VEGFR-2, but not VEGFR-1 or VEGFR-3. The antibody also works for immunofluorescent staining of VEGFR-2 in HUVEC cells.
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-VEGFR-2 antibody
ICC (Immunocytochemistry), ELISA, WB (Western Blot)
Immunogen
A synthetic peptide (coupled to carrier protein) corresponding to amino acids 1304 to 1317 in human VEGFR-2. This sequence has high homology to rat and mouse VEGFR-2, and no significant homology to other VEGFR family members.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
WB (Western Blot)
(Left: Western blot image of HUVEC cells untreated (-) or treated with pervanadate (1mM) for 30 min. (+). Right: Western blot image of GST-recombinant VEGFR-2 kinase without (-) or with (+) akaline phosphatase treatment. Both sets of blots were probed with rabbit polyclonal anti-VEGFR-2 (a.a. 1304-1317) or anti-VEGFR-2 (Tyr-801).)
WB (Western Blot)
(Left: Western blot image of HUVEC cells untreated (-) or treated with pervanadate (1mM) for 30 min. (+). Right: Western blot image of GST-recombinant VEGFR-2 kinase without (-) or with (+) akaline phosphatase treatment. Both sets of blots were probed with rabbit polyclonal anti-VEGFR-2 (a.a. 1304-1317) or anti-VEGFR-2 (Tyr-801).)
WB (Western Blot)
(Western blot image of GST-recombinant human VEGFR-1 (89kDa), VEGFR-2 (110kDa), and VEGFR-3 (86kDa) C-terminal regions. The blots were probed with rabbit polyclonal anti-VEGFR-2 (a.a. 1304-1317), anti-VEGFR-2 (Tyr-801, conserved site), and anti-VEGFR-3 (a.a. 1285-1298).)
WB (Western Blot)
(Western blot image of GST-recombinant human VEGFR-1 (89kDa), VEGFR-2 (110kDa), and VEGFR-3 (86kDa) C-terminal regions. The blots were probed with rabbit polyclonal anti-VEGFR-2 (a.a. 1304-1317), anti-VEGFR-2 (Tyr-801, conserved site), and anti-VEGFR-3 (a.a. 1285-1298).)
Related Product Information for anti-VEGFR-2 antibody
Vascular endothelial growth factor receptor-2 (VEGFR-2/Flk-1/KDR) is the primary receptor for VEGF in endothelial cells. Other VEGFR family members, VEGFR-1 (Flt-1) and VEGFR-3 (Flt-4), can also transduce the intracellular signals of VEGF. However, the role of VEGFR-1 is observed mainly during embryonic angiogenesis and VEGFR-3 signaling may be restricted to specific types of endothelial cells. Major autophosphorylation sites of VEGFR-2 are located in the kinase insert domain (Tyr-951/996) and in the tyrosine kinase catalytic domain (Tyr-1054/1059). Other sites, Tyr-1175 and Tyr-1212 provide docking sites for downstream signaling molecules. Activation of VEGFR-2 also phosphorylates Tyr-801, leading to PI3-kinase-Akt activation and increases in endothelial nitric oxide synthase activity. Phosphorylation of mutliple sites in VEGFR-2 is required for downstream activation of several signaling pathways that control proliferation, chemotaxis, and sprouting during angiogenesis.
References
Garcia Blanes, M. et al. (2007) J Biol. Chem. 282(14):10660.
Kiselyov, A. et al. (2007) Expert Opin. Investig. Drugs 16(1):83.
Robinson, C.J. & Stringer, S.E. (2001) J. Cell Sci.114:853.
Meyer, M. et al. (1999) EMBO J. 18:363.
Dougher-Vermazen, M. et al. (1994) Bioch Biophys Res Com. 205:728.
Kiselyov, A. et al. (2007) Expert Opin. Investig. Drugs 16(1):83.
Robinson, C.J. & Stringer, S.E. (2001) J. Cell Sci.114:853.
Meyer, M. et al. (1999) EMBO J. 18:363.
Dougher-Vermazen, M. et al. (1994) Bioch Biophys Res Com. 205:728.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
151,527 Da
NCBI Official Full Name
vascular endothelial growth factor receptor 2
NCBI Official Synonym Full Names
kinase insert domain receptor (a type III receptor tyrosine kinase)
NCBI Official Symbol
KDR
NCBI Official Synonym Symbols
FLK1; CD309; VEGFR; VEGFR2
NCBI Protein Information
vascular endothelial growth factor receptor 2; soluble VEGFR2; fetal liver kinase 1; fetal liver kinase-1; protein-tyrosine kinase receptor Flk-1; tyrosine kinase growth factor receptor
UniProt Protein Name
Vascular endothelial growth factor receptor 2
UniProt Gene Name
KDR
UniProt Synonym Gene Names
FLK1; VEGFR2; VEGFR-2; FLK-1; KDR
UniProt Entry Name
VGFR2_HUMAN
Similar Products
Product Notes
The VEGFR-2 kdr (Catalog #AAA71730) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The VEGFR-2 (a.a.1304-1317) Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's VEGFR-2 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the VEGFR-2 kdr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VEGFR-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.